Temporal and Spatial Dynamics of Cerebral Immune Cell Accumulation in Stroke
Mathias Gelderblom,Frank Leypoldt,Karin Steinbach,Doerthe Behrens,Chi-Un Choe,Dominic A. Siler,Thiruma V. Arumugam,Ellen Orthey,Christian Gerloff,Eva Tolosa,Tim Magnus +10 more
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TLDR
The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.Abstract:
Background and Purpose— Ischemic stroke leads to significant morbidity and mortality in the Western world Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage The understanding of postischemic inflammation is very limited The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia–reperfusion injury model Methods— Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets Results— Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells DCs exhiread more
Citations
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Stroke-associated inflammation: is von Willebrand factor a 'bad guy'?
TL;DR: A novel mechanism of neutrophil recruitment to the ischemic brain through von Willebrand factor (VWF) expressed on the endothelial cells is demonstrated and suggests that inhibition of WPB release could suppress cerebral inflammation and consequently be protective against isChemic injury.
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Mesenchymal stem cell therapy for ischemic stroke: Novel insight into the crosstalk with immune cells
TL;DR: In this article , Mesenchymal stem cells (MSC) transplantation is proposed as a promising treatment for alleviating neurological deficits, as indicated by a great number of animal and clinical studies.
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Reduced perforin release from T cells as a mechanism underlying hypothermia‐mediated neuroprotection
Tomohiro Matsui,Yusuke Yoshida +1 more
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Dissertation
Optimization of cell-based therapy using an injectable hydrogel after stroke : MRI and histological study
TL;DR: Therapeutic approaches that can be applied beyond acute phase are urgently needed and the application of new brain imaging techniques can be relevant for the comprehension of acute stroke mechanism and for a more accurate identification of candidates for acute phase reperfusion therapies.
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Multiplexed mRNA analysis of brain-derived extracellular vesicles upon experimental stroke in mice reveals increased mRNA content with potential relevance to inflammation and recovery processes
Annika Bub,Santra Brenna,Malik Alawi,Paul Kügler,Yuqi Gui,Oliver Kretz,Hermann C. Altmeppen,Tim Magnus,Berta Puig +8 more
TL;DR: In this article , the mRNA content of brain-derived extracellular vesicles (BDEVs) isolated 72 hours after experimental stroke in mice and compared them to controls (shams) using nCounter ® Nanostring panels, with or without prior RNA isolation.
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