Temporal and Spatial Dynamics of Cerebral Immune Cell Accumulation in Stroke
Mathias Gelderblom,Frank Leypoldt,Karin Steinbach,Doerthe Behrens,Chi-Un Choe,Dominic A. Siler,Thiruma V. Arumugam,Ellen Orthey,Christian Gerloff,Eva Tolosa,Tim Magnus +10 more
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TLDR
The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.Abstract:
Background and Purpose— Ischemic stroke leads to significant morbidity and mortality in the Western world Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage The understanding of postischemic inflammation is very limited The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia–reperfusion injury model Methods— Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets Results— Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells DCs exhiread more
Citations
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Crosstalk between microglia and T cells contributes to brain damage and recovery after ischemic stroke
Sunwei Wang,He Zhang,Yun Xu +2 more
TL;DR: Extensive evidence supports a critical role for the crosstalk of microglia and T cells in the prognosis of brain injury after ischemic stroke, and the regulation of the c Crosstalk may provide a potential therapeutic target for improving the isChemic brain damage.
Journal ArticleDOI
P2Y6 receptor inhibition aggravates ischemic brain injury by reducing microglial phagocytosis
Ruoxue Wen,Hui Shen,Shuxian Huang,Liping Wang,Zongwei Li,Peng Peng,Muyassar Mamtilahun,Yaohui Tang,Fanxia Shen,Heng-Li Tian,Guo-Yuan Yang,Zhijun Zhang +11 more
TL;DR: Clearance of damaged cells and debris is beneficial for the functional recovery after ischemic brain injury, however, the specific phagocytic receptor that mediates microglialphagocytosis after isChemic stroke is unknown.
Journal ArticleDOI
Evolution of ischemic damage and behavioural deficit over 6 months after MCAo in the rat: Selecting the optimal outcomes and statistical power for multi-centre preclinical trials.
Sarah S. J. Rewell,Leonid Churilov,T. Kate Sidon,Elena Aleksoska,Susan F Cox,Malcolm R. Macleod,David W. Howells,David W. Howells +7 more
TL;DR: This study investigates the development of ischemic damage after thread occlusion MCAo in the rat, using histological and behavioural outcomes and the adhesive removal test to investigate the longevity of behavioural deficit after isChemic stroke in rats, and examines the practicality of using such measures as the primary outcome for future studies.
Journal ArticleDOI
Classification of Microglial Morphological Phenotypes Using Machine Learning.
Judith Leyh,Sabine Paeschke,Bianca Mages,Dominik Michalski,Marcin Nowicki,Ingo Bechmann,Karsten Winter +6 more
TL;DR: In this paper, a novel microglial classification method by morphological evaluation using a convolutional neuronal network on the basis of manually selected cells in addition to classical morphological parameters was described.
Journal ArticleDOI
CCR6 (CC Chemokine Receptor 6) Is Essential for the Migration of Detrimental Natural Interleukin-17-Producing γδ T Cells in Stroke.
Priyadharshini Arunachalam,Peter Ludewig,Patrick Melich,Thiruma V. Arumugam,Christian Gerloff,Immo Prinz,Tim Magnus,Mathias Gelderblom +7 more
TL;DR: It is demonstrated that therapeutic approaches targeting synergistic IL-17 and tumor necrosis factor-&agr; pathways in parallel offer additional neuroprotection in stroke, and brain-infiltrating IL-18–positive T cells belong to the subset of natural IL- 17–producing T cells.
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