Temporal and Spatial Dynamics of Cerebral Immune Cell Accumulation in Stroke
Mathias Gelderblom,Frank Leypoldt,Karin Steinbach,Doerthe Behrens,Chi-Un Choe,Dominic A. Siler,Thiruma V. Arumugam,Ellen Orthey,Christian Gerloff,Eva Tolosa,Tim Magnus +10 more
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TLDR
The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.Abstract:
Background and Purpose— Ischemic stroke leads to significant morbidity and mortality in the Western world Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage The understanding of postischemic inflammation is very limited The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia–reperfusion injury model Methods— Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets Results— Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells DCs exhiread more
Citations
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Cerebrovascular plasticity: Processes that lead to changes in the architecture of brain microvessels.
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Protective effect of Naoxintong against cerebral ischemia reperfusion injury in mice.
Jing Xue,Xiangjian Zhang,Cong Zhang,Ning Kang,Xiaoxia Liu,Jingying Yu,Nan Zhang,Hong Wang,Lan Zhang,Rong Chen,Lili Cui,Lina Wang,Xiaolu Wang +12 more
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Hypothermia and brain inflammation after cardiac arrest.
TL;DR: The underlying pathophysiology of ischemia-reperfusion brain injury with emphasis on the role of inflammatory mechanisms is reviewed and potential targets for immunomodulatory treatments and relevant effects of TH are discussed.
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Potential Microglia-Based Interventions for Stroke
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Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains
Hyun Ah Kim,Stephanie C. Whittle,Seyoung Lee,Hannah X Chu,Shenpeng R. Zhang,Zihui Wei,Thiruma V. Arumugam,Thiruma V. Arumugam,Thiruma V. Arumugam,Anthony Vinh,Grant R Drummond,Christopher G. Sobey +11 more
TL;DR: The data are consistent with the concept that functional outcome after stroke is dependent on the immune cell composition which develops following ischemic brain injury.
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