Temporal and Spatial Dynamics of Cerebral Immune Cell Accumulation in Stroke
Mathias Gelderblom,Frank Leypoldt,Karin Steinbach,Doerthe Behrens,Chi-Un Choe,Dominic A. Siler,Thiruma V. Arumugam,Ellen Orthey,Christian Gerloff,Eva Tolosa,Tim Magnus +10 more
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TLDR
The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.Abstract:
Background and Purpose— Ischemic stroke leads to significant morbidity and mortality in the Western world Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage The understanding of postischemic inflammation is very limited The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia–reperfusion injury model Methods— Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets Results— Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells DCs exhiread more
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Regulatory T Cell in Stroke: A New Paradigm for Immune Regulation
TL;DR: Evidence of FoxP3+CD25+CD4+ Tregs as potentially important immunomodulators in stroke pathogenesis is summarized and further investigations for possible immunotherapeutic strategies by modulating the quantity and/or functional effects of FoxTregs in stroke patients are highlighted.
Journal ArticleDOI
Inflammatory cell recruitment after experimental thromboembolic stroke in rats.
J. Lehmann,Wolfgang Härtig,A. Seidel,C. Füldner,Carsten Hobohm,Jens Grosche,Martin Krueger,Dominik Michalski +7 more
TL;DR: This study provides novel data on the local inflammatory response to experimental thromboembolic stroke using a rodent model of stroke that closely reflects human embolic ischemia and recommends that future stroke research should preferably use animal models with relevance for clinical translation.
Journal ArticleDOI
Non-invasive tracking of CD4+ T cells with a paramagnetic and fluorescent nanoparticle in brain ischemia
Wei-Na Jin,Xiaoxia Yang,Zhiguo Li,Minshu Li,Samuel Xiang-Yu Shi,Kristofer Wood,Qingwei Liu,Ying Fu,Wei Han,Yun Xu,Fu-Dong Shi,Qiang Liu +11 more
TL;DR: In this paper, an in-vivo imaging approach for sequential monitoring of brain-infiltrating CD4+ T cells in experimental ischemic stroke was described, where magnetic resonance imaging (MRI) or Xenogen imaging combined with labeling of SPIO-Molday ION Rhodamine-B (MIRB) can be used to monitor the dynamics of CD4(+) T cells.
Journal ArticleDOI
The Role of Gamma-Delta T Cells in Diseases of the Central Nervous System.
TL;DR: A review of the neuro-inflammatory and immune functions of γδ T cells is offered, intending to understand their roles in CNS diseases, which may be crucial for the development of novel clinical applications.
Journal ArticleDOI
Single-cell RNA-seq reveals the transcriptional landscape in ischemic stroke:
Kai Zheng,Kai Zheng,Lingmin Lin,Wei Jiang,Lin Chen,Xiyue Zhang,Qian Zhang,Ren Yi,Junwei Hao,Junwei Hao +9 more
TL;DR: In this paper, the authors employed single-cell RNA sequencing (scRNA-seq) to comprehensively map the cell populations in the mouse model of MCAO (middle cerebral artery occlusion).
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