Temporal and Spatial Dynamics of Cerebral Immune Cell Accumulation in Stroke
Mathias Gelderblom,Frank Leypoldt,Karin Steinbach,Doerthe Behrens,Chi-Un Choe,Dominic A. Siler,Thiruma V. Arumugam,Ellen Orthey,Christian Gerloff,Eva Tolosa,Tim Magnus +10 more
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TLDR
The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.Abstract:
Background and Purpose— Ischemic stroke leads to significant morbidity and mortality in the Western world Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage The understanding of postischemic inflammation is very limited The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia–reperfusion injury model Methods— Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets Results— Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells DCs exhiread more
Citations
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Construction of bionanoparticles based on Angelica polysaccharides for the treatment of stroke.
TL;DR: Inspired by the ability of macrophage membranes to cross the blood-brain barrier, this article prepared amphiphilic nanoparticles by linking Angelica polysaccharide (APS) and Ethyl ferulate (EF) using oxalate bond (OL) as the linker arm, with an inner core encapsulated with Tetramethylpyrazine (TMP).
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Spatial and Temporal MRI Profile of Ischemic Tissue after the Acute Stages of a Permanent Mouse Model of Stroke
Bogaert-Buchmann A,Poittevin M,Po C,Dupont D,Catherine Sebrié,Yutaka Tomita,Alexy Tran-Dinh,Jacques Seylaz,Elisabeth Pinard,Philippe Meric,Nathalie Kubis,Brigitte Gillet +11 more
TL;DR: MRI parameters of microglial cells dynamics are provided for the first time, allowing its non-invasive monitoring during the chronic stages of a stroke, and could be particularly interesting in regards to emerging anti-inflammatory stroke therapies.
Journal ArticleDOI
Monocyte, neutrophil, and whole blood transcriptome dynamics following ischemic stroke
Paulina Carmona-Mora,Bodie Knepp,Glen C. Jickling,Xinhua Zhan,Marisa Hakoupian,Heather Hull,Noor Alomar,Hajar Amini,Frank R. Sharp,Boryana Stamova,Bradley P. Ander +10 more
TL;DR: In this paper , the temporal dynamics of gene expression after ischemic stroke were analyzed with RNA-seq as a function of time and etiology after stroke and differentially expression analyses were performed at 0-24 h, 24-48 h, and >48 h following stroke.
Book ChapterDOI
Platelets in Acute Ischemic Stroke
TL;DR: In experimental stroke, blocking of platelet GPIbα or GPVI, as well as reducing circulating VWF, dramatically improved stroke outcome by protecting the microvasculature during reperfusion and in addition accelerated recanalization during thrombolysis.
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Bim Deletion Reduces Functional Deficits Following Ischemic Stroke in Association with Modulation of Apoptosis and Inflammation
Jason Glab,Hamsa Puthalakath,Shenpeng R. Zhang,Antony Vinh,Grant R Drummond,Christopher G. Sobey,T. Michael De Silva,Hyun Ah Kim +7 more
TL;DR: In this article , the effect of Bim expression on post-stroke functional outcomes, brain injury and inflammatory mechanisms was investigated in wild type (WT) and Bim-deficient mice undergoing 1-h middle cerebral artery occlusion (MCAO) followed by 23 h of reperfusion.
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