Temporal and Spatial Dynamics of Cerebral Immune Cell Accumulation in Stroke
Mathias Gelderblom,Frank Leypoldt,Karin Steinbach,Doerthe Behrens,Chi-Un Choe,Dominic A. Siler,Thiruma V. Arumugam,Ellen Orthey,Christian Gerloff,Eva Tolosa,Tim Magnus +10 more
Reads0
Chats0
TLDR
The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.Abstract:
Background and Purpose— Ischemic stroke leads to significant morbidity and mortality in the Western world Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage The understanding of postischemic inflammation is very limited The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia–reperfusion injury model Methods— Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets Results— Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells DCs exhiread more
Citations
More filters
Journal ArticleDOI
Importance of T Lymphocytes in Brain Injury, Immunodeficiency, and Recovery after Cerebral Ischemia:
TL;DR: Though considerable recent progress has been made, a better understanding of the roles of the different T-lymphocyte subpopulations and their temporal profile of damage versus repair will help to clarify whether T-LYmphocyte targeting may be a viable poststroke therapy for clinical use.
Journal ArticleDOI
FTY720 Ameliorates Acute Ischemic Stroke in Mice by Reducing Thrombo-Inflammation but Not by Direct Neuroprotection
Peter Kraft,Eva Göb,Michael K. Schuhmann,Kerstin Göbel,Carsten Deppermann,Ina Thielmann,Alexander M. Herrmann,Kristina Lorenz,Marc Brede,Guido Stoll,Sven G. Meuth,Bernhard Nieswandt,Waltraud Pfeilschifter,Christoph Kleinschnitz +13 more
TL;DR: Induction of lymphocytopenia and concomitant reduction of microvascular thrombosis are key modes of FTY720 action in stroke, and findings in Rag1−/− mice and cultured neurons argue against direct neuroprotective effects of F TY720.
Journal ArticleDOI
Molecular dialogs between the ischemic brain and the peripheral immune system: Dualistic roles in injury and repair
Chengrui An,Yejie Shi,Peiying Li,Xiaoming Hu,Xiaoming Hu,Xiaoming Hu,Yu Gan,Yu Gan,R. A. Stetler,R. A. Stetler,R. A. Stetler,Rehana K. Leak,Yanqin Gao,Bao-liang Sun,Ping Zheng,Jun Chen,Jun Chen,Jun Chen +17 more
TL;DR: Pharmacological strategies and cell-based therapies that target the dialog between the brain and peripheral immune system show promise as potential novel treatments for stroke.
Journal ArticleDOI
LPS preconditioning redirects TLR signaling following stroke: TRIF-IRF3 plays a seminal role in mediating tolerance to ischemic injury
Keri B. Vartanian,Susan L. Stevens,Brenda J. Marsh,Rebecca L. Williams-Karnesky,Nikola S. Lessov,Mary P. Stenzel-Poore +5 more
TL;DR: LPS preconditioning redirects TLR4 signaling in response to stroke through suppression of NFκB activity, enhanced IRF3 activity, and increased anti-inflammatory/type I IFN gene expression, which highlights a critical role for TRIF-IRF3 signaling as the governing mechanism in the neuroprotective response to strokes.
Journal ArticleDOI
Advances in Meningeal Immunity
Rejane Rua,Dorian B. McGavern +1 more
TL;DR: Advances in the understanding of the meningeal immune repertoire are discussed and insights into how this CNS barrier operates immunologically under conditions ranging from neurocognition to inflammatory diseases are provided.
References
More filters
Journal ArticleDOI
Pathobiology of ischaemic stroke: an integrated view
TL;DR: This article provides a framework that can be used to generate testable hypotheses and treatment strategies that are linked to the appearance of specific pathophysiological events within the ischaemic brain.
Journal ArticleDOI
Microglia: active sensor and versatile effector cells in the normal and pathologic brain
TL;DR: This review focuses on several key observations that illustrate the multi-faceted activities of microglia in the normal and pathologic brain.
Journal ArticleDOI
Taking dendritic cells into medicine
TL;DR: Some medical implications of DC biology that account for illness and provide opportunities for prevention and therapy are presented.
Journal ArticleDOI
The inflammatory response in stroke.
TL;DR: The role of specific cell types including leukocytes, endothelium, glia, microglia, the extracellular matrix and neurons, and mediators produced by inflammatory cells such as cytokines, chemokines, reactive oxygen species and arachidonic acid metabolites are reviewed.
Journal ArticleDOI
A synthetic glycolipid prevents autoimmune encephalomyelitis by inducing TH2 bias of natural killer T cells.
TL;DR: A synthetic glycolipid ligand for CD1d-restricted natural killer T (NKT) cells expressing the semi-invariant T-cell receptor (Vα14+) is preventive against EAE and targeting NKT cells with this ligand may be an attractive means for intervening in human autoimmune diseases such as multiple sclerosis.