Temporal and Spatial Dynamics of Cerebral Immune Cell Accumulation in Stroke
Mathias Gelderblom,Frank Leypoldt,Karin Steinbach,Doerthe Behrens,Chi-Un Choe,Dominic A. Siler,Thiruma V. Arumugam,Ellen Orthey,Christian Gerloff,Eva Tolosa,Tim Magnus +10 more
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TLDR
The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.Abstract:
Background and Purpose— Ischemic stroke leads to significant morbidity and mortality in the Western world Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage The understanding of postischemic inflammation is very limited The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia–reperfusion injury model Methods— Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets Results— Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells DCs exhiread more
Citations
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Targeting the Immune System for Ischemic Stroke.
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The Role of Circular RNAs in Cerebral Ischemic Diseases: Ischemic Stroke and Cerebral Ischemia/Reperfusion Injury
TL;DR: This chapter will firstly review the pathophysiology, underlying mechanisms, and current treatments of cerebral isChemic diseases including ischemic stroke and cerebral ischemia/reperfusion injury, and then the characteristics and function of circular RNAs will be outlined and the roles circularRNAs play in human diseases are introduced.
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Sex differences in astrocyte and microglia responses immediately following middle cerebral artery occlusion in adult mice.
TL;DR: It is posited that a sex difference in the presence of constitutive CD11b has a role in determining male and female microglia phagocytic responses to ischemia and these findings are critical to understanding potential sex differences in glial physiology as well as stroke pathobiology which are foundational for the development of future sex-specific stroke therapies.
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