Temporal and Spatial Dynamics of Cerebral Immune Cell Accumulation in Stroke
Mathias Gelderblom,Frank Leypoldt,Karin Steinbach,Doerthe Behrens,Chi-Un Choe,Dominic A. Siler,Thiruma V. Arumugam,Ellen Orthey,Christian Gerloff,Eva Tolosa,Tim Magnus +10 more
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TLDR
The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.Abstract:
Background and Purpose— Ischemic stroke leads to significant morbidity and mortality in the Western world Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage The understanding of postischemic inflammation is very limited The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia–reperfusion injury model Methods— Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets Results— Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells DCs exhiread more
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Recombinant tissue plasminogen activator enhances microglial cell recruitment after stroke in mice.
Sébastien Lenglet,Fabrizio Montecucco,Adam Denes,Graham Coutts,Emmanuel Pinteaux,François Mach,Karl Lothard Schaller,Yvan Gasche,Jean-Christophe Copin +8 more
TL;DR: It is reported for the first time that rtPA amplified microglia recruitment early after stroke in association with a rapid CCL3 production, which may take part in the higher susceptibility of rTPA-treated animals to reperfusion injury.
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Prospects and limitations of using endogenous neural stem cells for brain regeneration.
TL;DR: The endogenous NSCs are activated in response to disease or trauma, and produce new neurons and glia, suggesting they have the potential to regenerate damaged brain tissue while avoiding the above-mentioned problems.
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Inhibiting the Migration of M1 Microglia at Hyperacute Period Could Improve Outcome of tMCAO Rats.
TL;DR: To study whether inhibiting microglia migration to the ischemic boundary zone (IBZ) at the early phase could improve neurological outcomes after stroke, a large number of mice were fitted with EMTs to treat central giant cell granuloma.
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The Neuroprotective Effect of Genetic Mannose-binding Lectin Deficiency is not Sustained in the Sub-acute Phase of Stroke
Andrew F. Ducruet,Sergey A. Sosunov,Brad E. Zacharia,Justin W. Gorski,Mason L. Yeh,Peter A. DeRosa,Gregory H. Cohen,Paul R. Gigante,E. Sander Connolly +8 more
TL;DR: Development of a successful anti-complement neuroprotective strategy will require carefully tailored inhibition coupled with a greater understanding of the functional effects of complement activation during later phases of stroke recovery.
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Abstract WP277: Non-invasive Tracking of CD4+ T Cells With a Paramagnetic and Fluorescent Nanoparticle in Brain Ischemia
Wei-Na Jin,Xiaoxia Yang,Zhiguo Li,Minshu Li,Samuel Xiang-Yu Shi,Kristofer Wood,Qingwei Liu,Ying Fu,Wei Han,Yun Xu,Shi Fudong,Qiang Liu +11 more
TL;DR: The use of MIRB coupled with in vivo imaging as a valid method to track CD4+ T cells in ischemic brain injury is demonstrated and will facilitate future investigations to identify the dynamics and key spatiotemporal events for brain-infiltrating lymphocytes in CNS inflammatory diseases.
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