Temporal and Spatial Dynamics of Cerebral Immune Cell Accumulation in Stroke
Mathias Gelderblom,Frank Leypoldt,Karin Steinbach,Doerthe Behrens,Chi-Un Choe,Dominic A. Siler,Thiruma V. Arumugam,Ellen Orthey,Christian Gerloff,Eva Tolosa,Tim Magnus +10 more
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TLDR
The peculiar activation pattern and massive increase of antigen-presenting cells in temporal conjunction with regulatory cells might provide additional insight into poststroke immune regulation.Abstract:
Background and Purpose— Ischemic stroke leads to significant morbidity and mortality in the Western world Early reperfusion strategies remain the treatment of choice but can initiate and augment an inflammatory response causing secondary brain damage The understanding of postischemic inflammation is very limited The objectives of this study were to define the temporal and spatial infiltration of immune cell populations and their activation patterns in a murine cerebral ischemia–reperfusion injury model Methods— Transient middle cerebral artery occlusion was induced for 1 hour followed by 12-hour to 7-day reperfusion in C57/BL6 mice Immunohistochemistry and flow cytometry were used to quantify the infiltrating immune cell subsets Results— Accumulation of microglia and infiltration of the ischemic hemisphere by macrophages, lymphocytes, and dendritic cells (DCs) preceded the neutrophilic influx DCs were found to increase 20-fold and constituted a substantial proportion of infiltrating cells DCs exhiread more
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Postischemic brain infiltration of leukocyte subpopulations differs among murine permanent and transient focal cerebral ischemia models.
Wei Zhou,Arthur Liesz,Henrike Bauer,Clemens Sommer,Bernd Lahrmann,Nektarios A. Valous,Niels Grabe,Roland Veltkamp +7 more
TL;DR: Comparison of the post‐ischemic neuroinflammation among murine permanent and transient middle cerebral artery occlusion (MCAO) models shows Neuroinflammation is more pronounced after permanent electrocoagulatory MCAO compared with 30 minutes and 90 minutes filament‐M CAO.
Journal ArticleDOI
The Temporal Dynamics of Poststroke Neuroinflammation: A Longitudinal Diffusion Tensor Imaging–Guided PET Study with 11C-PK11195 in Acute Subcortical Stroke
Alexander Thiel,Basia A. Radlinska,Caroline Paquette,Michael Sidel,Jean-Paul Soucy,Ralf Schirrmacher,Jeffrey Minuk +6 more
TL;DR: DTI-guided 11C-PK11195 PET in acute subcortical stroke demonstrates differential temporal dynamics of local and remote AMG, which might contribute differently to clinical outcome.
Journal ArticleDOI
Cognitive Deficits and Delayed Neuronal Loss in a Mouse Model of Multiple Microinfarcts
M. Wang,Jeffrey J. Iliff,Yonghong Liao,Michael Chen,Matthew Shinseki,Arun Venkataraman,J. Cheung,Wei Wang,M. Nedergaard +8 more
TL;DR: The long-lasting gliosis, delayed neuronal loss, and demyelination suggest that the therapeutic window for microinfarcts may be much wider (perhaps days to weeks) than for larger strokes.
Journal ArticleDOI
Systemic augmentation of αB-crystallin provides therapeutic benefit twelve hours post-stroke onset via immune modulation
Ahmet Arac,Sara E. Brownell,Jonathan B. Rothbard,Charlene Chen,Rose M. Ko,Marta P. Pereira,Gregory W. Albers,Lawrence Steinman,Gary K. Steinberg +8 more
TL;DR: Cryab is an endogenous anti-inflammatory and neuroprotectant molecule produced after stroke, whose beneficial properties can be augmented when administered therapeutically after stroke.
Journal ArticleDOI
Purinergic signalling in brain ischemia
Felicita Pedata,Ilaria Dettori,Elisabetta Coppi,Alessia Melani,Irene Fusco,Renato Corradetti,Anna Maria Pugliese +6 more
TL;DR: Evidence up to now in literature indicate that A2A receptor antagonists provide protection centrally by reducing excitotoxicity, while agonists at A1 (and possibly also A2B and A3 receptors provide protection by controlling massive infiltration and neuroinflammation in the hours and days after brain ischemia.
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