Journal ArticleDOI
Transcriptional regulation by Polycomb group proteins.
TLDR
The current knowledge of the PRC complexes is discussed, how they are targeted to chromatin and how the high diversity of the PcG proteins allows these complexes to influence cell identity.Abstract:
Polycomb group (PcG) proteins function within Polycomb repressive complexes (PRCs), which modify histones and other proteins and silence target genes. This Review highlights new insights into the role of PcG proteins in gene regulation, specifically in controlling self-renewal and differentiation of embryonic stem cells, and into how PRCs are targeted to chromatin.read more
Citations
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Book ChapterDOI
Analysis of Endogenous Protein Interactions of Polycomb Group of Proteins in Mouse Embryonic Stem Cells.
Lluis Morey,Luciano Di Croce +1 more
TL;DR: This chapter describes several methods to study Polycomb architecture, and identification of novel interactors in both pluripotent and differentiating mouse embryonic stem cells.
Dissertation
The role of Polycomb-mediated epigenetic regulation in embryonic stem cell differentiation
TL;DR: Analysis of gene expression and DNA methylation levels in several PRC2 mutant ESC lines that maintained varying levels of H3K27me3 found that genes that function to specify other lineages failed to be repressed, suggesting thatPRC2 activity is necessary for regulating lineage fidelity.
Journal ArticleDOI
Recent advances in EZH2-based dual inhibitors in the treatment of cancers.
Xiaojuan Yang,Lu Xu,Li Yang +2 more
TL;DR: In this article , the authors discuss the theoretical basis for designing EZH2-based dual-target inhibitors, and also describe some in vitro and in vivo analysis results, and discuss the use of dual inhibitors of two different targets mediated by one individual molecule.
Posted ContentDOI
MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2
TL;DR: It is found that PRC2 (Polycomb repressive complex 2) and its associated histone mark, H3K27me3, is enriched at hundreds of miRNA-repressed genes, and miRNAs post-transcriptionally reinforce silencing ofPRC2-repression genes that are inefficiently repressed at the level of chromatin.
Journal ArticleDOI
On the Advent of Super-Resolution Microscopy in the Realm of Polycomb Proteins
Irene Nepita,Simonluca Piazza,Martina Ruglioni,Sofia Cristiani,Emanuele Bosurgi,Tiziano Salvadori,Giuseppe Vicidomini,Alberto Diaspro,Marco Castello,Andrea Cerase,Paolo Bianchini,Barbara Storti,Ranieri Bizzarri +12 more
TL;DR: A review of the current state-of-the-art super-resolution microscopy applied to polycomb proteins can be found in this paper , showing that the application of SRM to PcG activity and organization is still quite limited and mainly focused on the 3D assembly of polycomb-controlled genomic loci.
References
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Journal ArticleDOI
Crystal structure of the nucleosome core particle at 2.8 Å resolution
TL;DR: The X-ray crystal structure of the nucleosome core particle of chromatin shows in atomic detail how the histone protein octamer is assembled and how 146 base pairs of DNA are organized into a superhelix around it.
Journal ArticleDOI
High-resolution profiling of histone methylations in the human genome.
Artem Barski,Suresh Cuddapah,Kairong Cui,Tae-Young Roh,Dustin E. Schones,Zhibin Wang,Gang Wei,Iouri Chepelev,Keji Zhao +8 more
TL;DR: High-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology are generated.
Journal ArticleDOI
Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Noncoding RNAs
John L. Rinn,Michael Kertesz,Jordon K. Wang,Sharon L. Squazzo,Xiao Xu,Samantha A. Brugmann,L. Henry Goodnough,Jill A. Helms,Peggy J. Farnham,Eran Segal,Howard Y. Chang +10 more
TL;DR: The transcriptional landscape of the four human HOX loci is characterized at five base pair resolution in 11 anatomic sites and 231 HOX ncRNAs are identified that extend known transcribed regions by more than 30 kilobases, suggesting transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance.
Journal ArticleDOI
A gene complex controlling segmentation in Drosophila.
TL;DR: The wild-type and mutant segmentation patterns are consistent with an antero-posterior gradient in repressor concentration along the embryo and a proximo-distal gradient along the chromosome in the affinities for repressor of each gene's cis-regulatory element.
Journal ArticleDOI
Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression
Ahmad M. Khalil,Mitchell Guttman,Maite Huarte,Manuel Garber,Arjun Raj,Dianali Rivea Morales,Kelly Thomas,Aviva Presser,Bradley E. Bernstein,Alexander van Oudenaarden,Aviv Regev,Eric S. Lander,John L. Rinn +12 more
TL;DR: A model in which some lincRNAs guide chromatin-modifying complexes to specific genomic loci to regulate gene expression is proposed, and it is shown that siRNA-mediated depletion of certain linc RNAs associated with PRC2 leads to changes in gene expression.