Journal ArticleDOI
Transcriptional regulation by Polycomb group proteins.
TLDR
The current knowledge of the PRC complexes is discussed, how they are targeted to chromatin and how the high diversity of the PcG proteins allows these complexes to influence cell identity.Abstract:
Polycomb group (PcG) proteins function within Polycomb repressive complexes (PRCs), which modify histones and other proteins and silence target genes. This Review highlights new insights into the role of PcG proteins in gene regulation, specifically in controlling self-renewal and differentiation of embryonic stem cells, and into how PRCs are targeted to chromatin.read more
Citations
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Maintenance of leukemic cell identity by the activity of the Polycomb complex PRC1 in mice
Alessandra Rossi,Karin Johanna Ferrari,Andrea Piunti,Sriganesh Jammula,Fulvio Chiacchiera,Luca Mazzarella,Andrea Scelfo,Pier Giuseppe Pelicci,Pier Giuseppe Pelicci,Diego Pasini +9 more
TL;DR: It is genetically demonstrated that PRC1 activity and the deposition of H2AK119Ub are critical factors that maintain the undifferentiated identity of cancer cells, positively sustaining the progression of different types of leukemia.
Journal ArticleDOI
Concise Review: Geminin-A Tale of Two Tails: DNA Replication and Transcriptional/Epigenetic Regulation in Stem Cells.
Alexandra L. Patmanidi,Spyridon Champeris Tsaniras,Dimitris Karamitros,Christina Kyrousi,Zoi Lygerou,Stavros Taraviras +5 more
TL;DR: Current knowledge is summarized and new perspectives for the role of Geminin on transcriptional and epigenetic regulation, alongside with its regulatory activity in DNA replication and their implication in the regulation of stem and progenitor cell biology are given.
Journal ArticleDOI
An epigenetic view of B-cell disorders.
TL;DR: Understanding how specific epigenetic alterations contribute to the development of lymphomas, autoimmunity and EBV‐associated disorders is instrumental to develop novel therapeutic interventions for the cure of these often fatal pathologies.
Journal ArticleDOI
A Potent, Selective CBX2 Chromodomain Ligand and Its Cellular Activity During Prostate Cancer Neuroendocrine Differentiation
Sijie Wang,Aktan Alpsoy,Surbhi Sood,Sandra Carolina Ordonez-Rubiano,Alisha Dhiman,Yixing Sun,Guanming Jiao,Casey J. Krusemark,Emily C. Dykhuizen +8 more
TL;DR: In this paper, focused DNA encoded libraries (DELs) were used for the discovery of a selective CBX2 chromodomain probe, SW2_152F, which is cell permeable, selectively inhibits CBX 2 chromatin binding in cells, and blocks neuroendocrine differentiation of prostate cancer cell lines in response to androgen deprivation.
Journal ArticleDOI
The EZH2 SANT1 domain is a histone reader providing sensitivity to the modification state of the H4 tail
Tyler M. Weaver,Jiachen Liu,Katelyn E. Connelly,Chris Coble,Katayoun Varzavand,Emily C. Dykhuizen,Catherine A. Musselman +6 more
TL;DR: The first SANT domain (SANT1) of EZH2 is a histone binding domain with specificity for the histone H4 N-terminal tail and is structurally characterize and determined the molecular mechanism of binding to the H4 tail.
References
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Journal ArticleDOI
Crystal structure of the nucleosome core particle at 2.8 Å resolution
TL;DR: The X-ray crystal structure of the nucleosome core particle of chromatin shows in atomic detail how the histone protein octamer is assembled and how 146 base pairs of DNA are organized into a superhelix around it.
Journal ArticleDOI
High-resolution profiling of histone methylations in the human genome.
Artem Barski,Suresh Cuddapah,Kairong Cui,Tae-Young Roh,Dustin E. Schones,Zhibin Wang,Gang Wei,Iouri Chepelev,Keji Zhao +8 more
TL;DR: High-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology are generated.
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Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Noncoding RNAs
John L. Rinn,Michael Kertesz,Jordon K. Wang,Sharon L. Squazzo,Xiao Xu,Samantha A. Brugmann,L. Henry Goodnough,Jill A. Helms,Peggy J. Farnham,Eran Segal,Howard Y. Chang +10 more
TL;DR: The transcriptional landscape of the four human HOX loci is characterized at five base pair resolution in 11 anatomic sites and 231 HOX ncRNAs are identified that extend known transcribed regions by more than 30 kilobases, suggesting transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance.
Journal ArticleDOI
A gene complex controlling segmentation in Drosophila.
TL;DR: The wild-type and mutant segmentation patterns are consistent with an antero-posterior gradient in repressor concentration along the embryo and a proximo-distal gradient along the chromosome in the affinities for repressor of each gene's cis-regulatory element.
Journal ArticleDOI
Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression
Ahmad M. Khalil,Mitchell Guttman,Maite Huarte,Manuel Garber,Arjun Raj,Dianali Rivea Morales,Kelly Thomas,Aviva Presser,Bradley E. Bernstein,Alexander van Oudenaarden,Aviv Regev,Eric S. Lander,John L. Rinn +12 more
TL;DR: A model in which some lincRNAs guide chromatin-modifying complexes to specific genomic loci to regulate gene expression is proposed, and it is shown that siRNA-mediated depletion of certain linc RNAs associated with PRC2 leads to changes in gene expression.