Journal ArticleDOI
Transcriptional regulation by Polycomb group proteins.
TLDR
The current knowledge of the PRC complexes is discussed, how they are targeted to chromatin and how the high diversity of the PcG proteins allows these complexes to influence cell identity.Abstract:
Polycomb group (PcG) proteins function within Polycomb repressive complexes (PRCs), which modify histones and other proteins and silence target genes. This Review highlights new insights into the role of PcG proteins in gene regulation, specifically in controlling self-renewal and differentiation of embryonic stem cells, and into how PRCs are targeted to chromatin.read more
Citations
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Journal ArticleDOI
A chromatin-independent role of polycomb-like 1 to stabilize p53 and promote cellular quiescence
Gerard L. Brien,Evan Healy,Emilia Jerman,Eric Conway,Elisa Fadda,Darragh O'Donovan,Andrei V. Krivtsov,Alan M. Rice,Conor J. Kearney,Andrew Flaus,Simon S. McDade,Seamus J. Martin,Aoife McLysaght,David J. O'Connell,Scott A. Armstrong,Adrian P. Bracken +15 more
TL;DR: The functional bifurcation of PCL proteins is illustrated, which act in both a chromatin-dependent and a Chromatin-independent manner to regulate the INK4A and p53 pathways.
Journal ArticleDOI
Discovery and Characterization of a Cellular Potent Positive Allosteric Modulator of the Polycomb Repressive Complex 1 Chromodomain, CBX7
Kelsey N. Lamb,Daniel Bsteh,Sarah N. Dishman,Hagar F. Moussa,Huitao Fan,Jacob I. Stuckey,Jacqueline L. Norris,Stephanie H. Cholensky,Dongxu Li,Jingkui Wang,Cari A. Sagum,Benjamin Z. Stanton,Mark T. Bedford,Kenneth H. Pearce,Terry P. Kenakin,Dmitri Kireev,Gang Greg Wang,Lindsey I. James,Oliver Bell,Oliver Bell,Stephen V. Frye +20 more
TL;DR: The discovery and characterization of UNC4976 not only revealed the most cellularly potent PRC1-specific chemical probe to date, but also uncovers a potential mechanism of Polycomb regulation with implications for non-histone lysine methylated interaction partners.
Journal ArticleDOI
RNAi-dependent Polycomb repression controls transposable elements in Tetrahymena
Xiaolu Zhao,Jie Xiong,Fengbiao Mao,Yalan Sheng,Xiao Chen,Xiao Chen,Lifang Feng,Wen Dui,Wentao Yang,Aurélie Kapusta,Cédric Feschotte,Robert S. Coyne,Wei Miao,Shan Gao,Yifan Liu +14 more
TL;DR: In the protozoan Tetrahymena thermophila, germline-specific internally eliminated sequences (IESs) become transcriptionally activated in mutants deficient in the RNAi-dependent Polycomb repression pathway.
Journal ArticleDOI
Polycomb genes, miRNA, and their deregulation in B-cell malignancies.
TL;DR: The current understanding of PcG-mediated gene silencing, interplays of P cG with other epigenetic regulators such as miRNAs during B-cell differentiation and lymphomagenesis, and recent advancements in targeted strategies against Pcg are summarized as promising therapeutics for B- cell malignancies are summarized.
Journal ArticleDOI
Polycomb group RING finger proteins 3/5 activate transcription via an interaction with the pluripotency factor Tex10 in embryonic stem cells
Wukui Zhao,Yikai Huang,Jingzi Zhang,Mengjie Liu,Haijing Ji,Congcong Wang,Ning Cao,Chao-Jun Li,Yin Xia,Qing Jiang,Jinzhong Qin +10 more
TL;DR: These findings establish Pcgf3/5 as transcriptional activators that interact with testis-expressed 10 and p300 in ES cells and point to redundant activity of Pc gf4/5 in pluripotency maintenance and are essential for regulating global levels of the histone modifier H2AK119ub1 in ES Cells.
References
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Journal ArticleDOI
Crystal structure of the nucleosome core particle at 2.8 Å resolution
TL;DR: The X-ray crystal structure of the nucleosome core particle of chromatin shows in atomic detail how the histone protein octamer is assembled and how 146 base pairs of DNA are organized into a superhelix around it.
Journal ArticleDOI
High-resolution profiling of histone methylations in the human genome.
Artem Barski,Suresh Cuddapah,Kairong Cui,Tae-Young Roh,Dustin E. Schones,Zhibin Wang,Gang Wei,Iouri Chepelev,Keji Zhao +8 more
TL;DR: High-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology are generated.
Journal ArticleDOI
Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Noncoding RNAs
John L. Rinn,Michael Kertesz,Jordon K. Wang,Sharon L. Squazzo,Xiao Xu,Samantha A. Brugmann,L. Henry Goodnough,Jill A. Helms,Peggy J. Farnham,Eran Segal,Howard Y. Chang +10 more
TL;DR: The transcriptional landscape of the four human HOX loci is characterized at five base pair resolution in 11 anatomic sites and 231 HOX ncRNAs are identified that extend known transcribed regions by more than 30 kilobases, suggesting transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance.
Journal ArticleDOI
A gene complex controlling segmentation in Drosophila.
TL;DR: The wild-type and mutant segmentation patterns are consistent with an antero-posterior gradient in repressor concentration along the embryo and a proximo-distal gradient along the chromosome in the affinities for repressor of each gene's cis-regulatory element.
Journal ArticleDOI
Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression
Ahmad M. Khalil,Mitchell Guttman,Maite Huarte,Manuel Garber,Arjun Raj,Dianali Rivea Morales,Kelly Thomas,Aviva Presser,Bradley E. Bernstein,Alexander van Oudenaarden,Aviv Regev,Eric S. Lander,John L. Rinn +12 more
TL;DR: A model in which some lincRNAs guide chromatin-modifying complexes to specific genomic loci to regulate gene expression is proposed, and it is shown that siRNA-mediated depletion of certain linc RNAs associated with PRC2 leads to changes in gene expression.