Journal ArticleDOI
Transcriptional regulation by Polycomb group proteins.
TLDR
The current knowledge of the PRC complexes is discussed, how they are targeted to chromatin and how the high diversity of the PcG proteins allows these complexes to influence cell identity.Abstract:
Polycomb group (PcG) proteins function within Polycomb repressive complexes (PRCs), which modify histones and other proteins and silence target genes. This Review highlights new insights into the role of PcG proteins in gene regulation, specifically in controlling self-renewal and differentiation of embryonic stem cells, and into how PRCs are targeted to chromatin.read more
Citations
More filters
Journal ArticleDOI
PRC1 Stabilizes Cardiac Contraction by Regulating Cardiac Sarcomere Assembly and Cardiac Conduction System Construction.
TL;DR: In this article, a loss-of-function zebrafish line was generated by using the CRISPR/Cas9 system targeting rnf2, a gene encoding the core subunit shared by all PRC1 subfamilies.
Posted ContentDOI
Inheritance of Repressed Chromatin Domains during S-phase Requires the Histone Chaperone NPM1
Thelma M. Escobar,Thelma M. Escobar,Thelma M. Escobar,Jia Ray Yu,Jia Ray Yu,Sanxiong Liu,Sanxiong Liu,Kimberly Lucero,Kimberly Lucero,Nikita Vasilyev,Nikita Vasilyev,Evgeny Nudler,Evgeny Nudler,Danny Reinberg,Danny Reinberg +14 more
TL;DR: In this article, NPM1, a histone H3/H4 chaperone, was shown to foster epigenetic inheritance from parental repressed chromatin during DNA replication.
Journal ArticleDOI
Dnmt1 activity is dispensable in δ-cells but is essential for α-cell homeostasis.
TL;DR: It is found that loss of Dnmt1 does not enhance the conversion of α- or δ-cells toward a β-like fate, and Ezh2 inactivation does not enhanced α-cell plasticity, and, contrary to what is observed in β-cells, does not impair α- cell proliferation.
Journal ArticleDOI
A Chromatin-Focused siRNA Screen for Regulators of p53-Dependent Transcription.
TL;DR: This work utilized an siRNA library designed against predicted chromatin regulatory proteins, and identified known and novel chromatin-related factors that affect both global p53-dependent transcription and gene-specific regulators of p53 transcriptional activation.
Dissertation
Rôle des facteurs de transcription Eos et Helios dans la biologie des cellules T régulatrices
TL;DR: In this paper, the authors decouvertes indiquent que la perte combinee d'Eos et d’Helios n’a pas d'effet sur la biologie des cellules Treg.
References
More filters
Journal ArticleDOI
Crystal structure of the nucleosome core particle at 2.8 Å resolution
TL;DR: The X-ray crystal structure of the nucleosome core particle of chromatin shows in atomic detail how the histone protein octamer is assembled and how 146 base pairs of DNA are organized into a superhelix around it.
Journal ArticleDOI
High-resolution profiling of histone methylations in the human genome.
Artem Barski,Suresh Cuddapah,Kairong Cui,Tae-Young Roh,Dustin E. Schones,Zhibin Wang,Gang Wei,Iouri Chepelev,Keji Zhao +8 more
TL;DR: High-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology are generated.
Journal ArticleDOI
Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Noncoding RNAs
John L. Rinn,Michael Kertesz,Jordon K. Wang,Sharon L. Squazzo,Xiao Xu,Samantha A. Brugmann,L. Henry Goodnough,Jill A. Helms,Peggy J. Farnham,Eran Segal,Howard Y. Chang +10 more
TL;DR: The transcriptional landscape of the four human HOX loci is characterized at five base pair resolution in 11 anatomic sites and 231 HOX ncRNAs are identified that extend known transcribed regions by more than 30 kilobases, suggesting transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance.
Journal ArticleDOI
A gene complex controlling segmentation in Drosophila.
TL;DR: The wild-type and mutant segmentation patterns are consistent with an antero-posterior gradient in repressor concentration along the embryo and a proximo-distal gradient along the chromosome in the affinities for repressor of each gene's cis-regulatory element.
Journal ArticleDOI
Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression
Ahmad M. Khalil,Mitchell Guttman,Maite Huarte,Manuel Garber,Arjun Raj,Dianali Rivea Morales,Kelly Thomas,Aviva Presser,Bradley E. Bernstein,Alexander van Oudenaarden,Aviv Regev,Eric S. Lander,John L. Rinn +12 more
TL;DR: A model in which some lincRNAs guide chromatin-modifying complexes to specific genomic loci to regulate gene expression is proposed, and it is shown that siRNA-mediated depletion of certain linc RNAs associated with PRC2 leads to changes in gene expression.