Journal ArticleDOI
Transcriptional regulation by Polycomb group proteins.
TLDR
The current knowledge of the PRC complexes is discussed, how they are targeted to chromatin and how the high diversity of the PcG proteins allows these complexes to influence cell identity.Abstract:
Polycomb group (PcG) proteins function within Polycomb repressive complexes (PRCs), which modify histones and other proteins and silence target genes. This Review highlights new insights into the role of PcG proteins in gene regulation, specifically in controlling self-renewal and differentiation of embryonic stem cells, and into how PRCs are targeted to chromatin.read more
Citations
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Journal ArticleDOI
Core Components of the Nuclear Pore Bind Distinct States of Chromatin and Contribute to Polycomb Repression.
Alejandro Gozalo,Ashley A. Duke,Yemin Lan,Pau Pascual-Garcia,Jessica A. Talamas,Son C. Nguyen,Parisha P. Shah,Rajan Jain,Eric F. Joyce,Maya Capelson +9 more
TL;DR: This investigation uncovered differential binding of these NPC subunits, where Nup107 preferentially targets active genes while Nup93 associates primarily with Polycomb-silenced regions, and lend to a model where different nuclear pores bind different types of chromatin via interactions with specific NPC sub-complexes, and a subset of Polycomb domains is stabilized by interactions with N up93.
Journal ArticleDOI
USP21 deubiquitinase promotes pancreas cancer cell stemness via Wnt pathway activation
Pingping Hou,Xingdi Ma,Qiang Zhang,Chang-Jiun Wu,Wenting Liao,Wenting Liao,Jun Li,Huamin Wang,Jun Zhao,Xin Zhou,Carolyn Guan,Jeffery Ackroyd,Shan Jiang,Jianhua Zhang,Denise J. Spring,Y. Alan Wang,Ronald A. DePinho +16 more
TL;DR: Using in vivo approaches, USP21 deubiquitinates TCF7, a member of the Wnt pathway, promoting pancreatic cancer cell proliferation and stemness, and is identified as one of the most commonly amplified genes in PDAC.
Journal ArticleDOI
BMI1 regulates PRC1 architecture and activity through homo- and hetero-oligomerization.
Felicia Gray,Hyo Je Cho,Shirish Shukla,Shihan He,Ashley Harris,Bohdan Boytsov,Łukasz Jaremko,Mariusz Jaremko,Borries Demeler,Elizabeth R. Lawlor,Jolanta Grembecka,Tomasz Cierpicki +11 more
TL;DR: It is demonstrated that both the interaction of BMI1 with polyhomeotic proteins and homo-oligomerization via UBL domain are necessary for H2A ubiquitination activity of PRC1 and for clonogenic potential of U2OS cells.
Journal ArticleDOI
PCGF5 is required for neural differentiation of embryonic stem cells.
Mingze Yao,Xueke Zhou,Jiajian Zhou,Shixin Gong,Gongcheng Hu,Jiao Li,Kaimeng Huang,Ping Lai,Guang Shi,Andrew P. Hutchins,Hao Sun,Huating Wang,Hongjie Yao +12 more
TL;DR: It is reported that PCGF5, one of six PCGF paralogs, is an important requirement in the differentiation of mouse embryonic stem cells (mESCs) towards a neural cell fate and the findings reveal a critical context-specific function forPCGF5 in directing PRC1 to control cell fate.
Journal ArticleDOI
Ageing as developmental decay: insights from p16(INK4a.).
TL;DR: The hypothesis that ageing might be primarily driven by gradual functional decay of developmental pathways is presented and the role of p16(INK4a) in ageing is summarised and the current knowledge on p16 (INK4A) regulation is summarized.
References
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Journal ArticleDOI
Crystal structure of the nucleosome core particle at 2.8 Å resolution
TL;DR: The X-ray crystal structure of the nucleosome core particle of chromatin shows in atomic detail how the histone protein octamer is assembled and how 146 base pairs of DNA are organized into a superhelix around it.
Journal ArticleDOI
High-resolution profiling of histone methylations in the human genome.
Artem Barski,Suresh Cuddapah,Kairong Cui,Tae-Young Roh,Dustin E. Schones,Zhibin Wang,Gang Wei,Iouri Chepelev,Keji Zhao +8 more
TL;DR: High-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology are generated.
Journal ArticleDOI
Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Noncoding RNAs
John L. Rinn,Michael Kertesz,Jordon K. Wang,Sharon L. Squazzo,Xiao Xu,Samantha A. Brugmann,L. Henry Goodnough,Jill A. Helms,Peggy J. Farnham,Eran Segal,Howard Y. Chang +10 more
TL;DR: The transcriptional landscape of the four human HOX loci is characterized at five base pair resolution in 11 anatomic sites and 231 HOX ncRNAs are identified that extend known transcribed regions by more than 30 kilobases, suggesting transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance.
Journal ArticleDOI
A gene complex controlling segmentation in Drosophila.
TL;DR: The wild-type and mutant segmentation patterns are consistent with an antero-posterior gradient in repressor concentration along the embryo and a proximo-distal gradient along the chromosome in the affinities for repressor of each gene's cis-regulatory element.
Journal ArticleDOI
Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression
Ahmad M. Khalil,Mitchell Guttman,Maite Huarte,Manuel Garber,Arjun Raj,Dianali Rivea Morales,Kelly Thomas,Aviva Presser,Bradley E. Bernstein,Alexander van Oudenaarden,Aviv Regev,Eric S. Lander,John L. Rinn +12 more
TL;DR: A model in which some lincRNAs guide chromatin-modifying complexes to specific genomic loci to regulate gene expression is proposed, and it is shown that siRNA-mediated depletion of certain linc RNAs associated with PRC2 leads to changes in gene expression.