FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism
Jennifer C. Darnell,Sarah J. Van Driesche,Chaolin Zhang,Ka Ying Sharon Hung,Aldo Mele,Claire E. Fraser,Elizabeth F. Stone,Cynthia Chen,John J. Fak,Sung Wook Chi,Donny D. Licatalosi,Joel D. Richter,Robert B. Darnell,Robert B. Darnell +13 more
TLDR
A brain polyribosome-programmed translation system is developed, revealing that FMRP reversibly stalls ribosomes specifically on its target mRNAs and suggests multiple targets for clinical intervention in FXS and ASD.About:
This article is published in Cell.The article was published on 2011-07-22 and is currently open access. It has received 1861 citations till now. The article focuses on the topics: FMR1 & RNA-binding protein.read more
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Journal ArticleDOI
Fragile X Mental Retardation Protein Bidirectionally Controls Dendritic Ih in a Cell Type-Specific Manner between Mouse Hippocampus and Prefrontal Cortex.
Federico Brandalise,Brian E. Kalmbach,Preeti Mehta,Olivia Thornton,Daniel Johnston,Boris V. Zemelman,Darrin H. Brager +6 more
TL;DR: Replacing Fragile X Mental Retardation Protein with FMRP in adult CA1 and L5 PFC neurons regulates the number of functional dendritic HCN channels in a cell type-specific manner.
Posted ContentDOI
Genetic identification Of brain cell types underlying schizophrenia
Nathan G. Skene,Julien Bryois,Trygve E. Bakken,Gerome Breen,James J. Crowley,Helena Gaspar,Paola Giusti-Rodríguez,Rebecca D. Hodge,Jeremy A. Miller,Ana B. Muñoz-Manchado,Michael Conlon O'Donovan,Michael J. Owen,Antonio F. Pardiñas,Jesper Ryge,James T.R. Walters,Sten Linnarsson,Ed S. Lein,Patrick F. Sullivan,Jens Hjerling-Leffler +18 more
TL;DR: The results provide a parsimonious explanation: the common-variant genetic results for schizophrenia point at a limited set of neurons, and the gene sets point to the same cells.
Book
Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing
TL;DR: Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing aims to collect in a single volume all aspects that should be taken into account when HTS technologies are being incorporated into a research project and the reasons behind them.
Journal ArticleDOI
Cataloguing and Selection of mRNAs Localized to Dendrites in Neurons and Regulated by RNA-Binding Proteins in RNA Granules
Rie Ohashi,Nobuyuki Shiina +1 more
TL;DR: These findings provide insights into the mechanistic basis of protein-synthesis-dependent synaptic plasticity and memory formation and contribute to future efforts to understand the physiological implications of local regulation of dendritic mRNAs in neurons.
Journal ArticleDOI
UPF1 Governs Synaptic Plasticity through Association with a STAU2 RNA Granule.
Tyson E. Graber,Erika Freemantle,Mina N. Anadolu,Sarah Hébert-Seropian,Robyn L. MacAdam,Unkyung Shin,Huy-Dung Hoang,Tommy Alain,Jean-Claude Lacaille,Wayne S. Sossin +9 more
TL;DR: It is demonstrated that UPF1, known to stall peptide release during nonsense-mediated RNA decay, is critical for assembly of stalled polysomes in rat hippocampal neurons derived from embryos of either sex, and a new role is described for the ATP-dependent helicase UpF1 and its interaction with the RNA localization protein STAU2 in mediating mGluR-LTD through the regulation of mRNA translation complexes stalled at the level of elongation and/or termination.
References
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Journal ArticleDOI
Controlling the false discovery rate: a practical and powerful approach to multiple testing
Yoav Benjamini,Yosef Hochberg +1 more
TL;DR: In this paper, a different approach to problems of multiple significance testing is presented, which calls for controlling the expected proportion of falsely rejected hypotheses -the false discovery rate, which is equivalent to the FWER when all hypotheses are true but is smaller otherwise.
Journal ArticleDOI
Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources.
TL;DR: By following this protocol, investigators are able to gain an in-depth understanding of the biological themes in lists of genes that are enriched in genome-scale studies.
Biometery: The principles and practice of statistics in biological research
TL;DR: In this paper, the authors present a model for the analysis of variance in a single-classification and two-way and multiway analysis of Variance with the assumption of correlation.
Book
Biometry: The Principles and Practice of Statistics in Biological Research
Robert R. Sokal,F. James Rohlf +1 more
TL;DR: In this paper, the authors present a model for the analysis of variance in a single-classification and two-way and multiway analysis of Variance with the assumption of correlation.
Journal ArticleDOI
Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray Experiments
TL;DR: The hierarchical model of Lonnstedt and Speed (2002) is developed into a practical approach for general microarray experiments with arbitrary numbers of treatments and RNA samples and the moderated t-statistic is shown to follow a t-distribution with augmented degrees of freedom.
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