FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism
Jennifer C. Darnell,Sarah J. Van Driesche,Chaolin Zhang,Ka Ying Sharon Hung,Aldo Mele,Claire E. Fraser,Elizabeth F. Stone,Cynthia Chen,John J. Fak,Sung Wook Chi,Donny D. Licatalosi,Joel D. Richter,Robert B. Darnell,Robert B. Darnell +13 more
TLDR
A brain polyribosome-programmed translation system is developed, revealing that FMRP reversibly stalls ribosomes specifically on its target mRNAs and suggests multiple targets for clinical intervention in FXS and ASD.About:
This article is published in Cell.The article was published on 2011-07-22 and is currently open access. It has received 1861 citations till now. The article focuses on the topics: FMR1 & RNA-binding protein.read more
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Studies on Aplysia neurons suggest treatments for chronic human disorders.
TL;DR: This research on this simple molluscan nervous system may lead to new therapeutic approaches for spinal cord injury, Fragile X syndrome, and genetic learning deficits more generally.
Journal ArticleDOI
RNA-Binding Specificity of the Human Fragile X Mental Retardation Protein.
Youssi M. Athar,Simpson Joseph +1 more
TL;DR: A new method called Motif Identification by Analysis of Simple sequences (MIDAS) to identify single-stranded RNA sequences bound by KH domains is developed, which finds that the FMRP KH0, KH1, and KH2 domains bind weakly to the ssRNA sequences suggesting that they may have evolved to bind more complex RNA structures.
Journal ArticleDOI
The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches.
TL;DR: In this paper, a function-based ontology of stable RNPs is proposed and discussed how Grad-seq and related approaches transformed our perspective of bacterial and eukaryotic ribonucleoproteins by guiding the discovery of new RNA-binding proteins and unusual classes of noncoding RNAs.
Journal ArticleDOI
Where Environment Meets Cognition: A Focus on Two Developmental Intellectual Disability Disorders
TL;DR: Epigenetic studies of IDDs, focusing on DS and FXS, are discussed, and the use of epidrugs in combinatorial therapies for IDDs is discussed.
Journal ArticleDOI
Optimization of ribosome profiling using low-input brain tissue from fragile X syndrome model mice
TL;DR: The data reveal that optimal RNase digestion is essential to ensure high quality and reproducibility of ribosome profiling for low-input brain tissue and establish GC content and Ribosome footprint length as quality control metrics forRNase digestion.
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Biometry: The Principles and Practice of Statistics in Biological Research
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TL;DR: In this paper, the authors present a model for the analysis of variance in a single-classification and two-way and multiway analysis of Variance with the assumption of correlation.
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