How do lncRNAs regulate transcription
TLDR
Recent progress in elucidating the molecular mechanisms by which lncRNAs modulate gene expression is reviewed, including the act of lnc RNA transcription rather than the lncRNA product that appears to be regulatory.Abstract:
It has recently become apparent that RNA, itself the product of transcription, is a major regulator of the transcriptional process. In particular, long noncoding RNAs (lncRNAs), which are so numerous in eukaryotes, function in many cases as transcriptional regulators. These RNAs function through binding to histone-modifying complexes, to DNA binding proteins (including transcription factors), and even to RNA polymerase II. In other cases, it is the act of lncRNA transcription rather than the lncRNA product that appears to be regulatory. We review recent progress in elucidating the molecular mechanisms by which lncRNAs modulate gene expression and future opportunities in this research field.read more
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Enhancement of protein translation by CRISPR/dCasRx coupled with SINEB2 repeat of noncoding RNAs
TL;DR: The CRISPR/dCasRx-SINEB2 technology was developed in this article by coupling the sgRNA of a catalytically inactive Type VI-D Cas13 enzyme to an integrated SINEB 2 domain of uchl1 lncRNA that promotes the translation of targeted mRNA.
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Control of Intra-Thymic αβ T Cell Selection and Maturation by H3K27 Methylation and Demethylation.
TL;DR: The present review discusses the importance of methylation and chromatin structure mechanisms during intrathymic αβ T cell selection and late differentiation in eukaryotes.
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Emerging role of lncRNAs in drug resistance mechanisms in head and neck squamous cell carcinoma
José A. Peña-Flores,Mercedes Bermúdez,Rosalío Ramos-Payán,C. E. Villegas-Mercado,Uriel Soto-Barreras,Daniela Muela-Campos,Alexis Álvarez-Ramírez,Brenda Perez-Aguirre,Ana Larrinua-Pacheco,César López-Camarillo,J.A. López-Gutierrez,Julio Carlos Garnica-Palazuelos,Marvin E. Estrada-Macías,J. Cota-Quintero,Andrés A. Barraza-Gómez +14 more
TL;DR: Current knowledge about the implication of lncRNAs in drug resistance mechanisms in HNSCC is provided and an improvement in diagnostic and prognostic tools could promote an advance in targeted and specific therapies in precision oncology.
Journal ArticleDOI
Transcription termination sequences support the expression of transgene product secreted with milk.
Alexey V. Deykin,M. V. Tikhonov,V A Kalmykov,Igor V. Korobko,Pavel Georgiev,Oksana Maksimenko +5 more
TL;DR: Comparison of the ability of well-studied chicken chromatin insulator HS4 and bidirectional transcription terminators from the human genome to support high-level expression of the firefly luciferase gene (Fluc) under control of the previously characterized goat β-casein gene promoter suggests that transcriptional terminators, similar to the HS4 insulator, can be used to improve the reporter gene expression in transgenic animals.
Journal ArticleDOI
Construction and analysis of mRNA, lncRNA, and transcription factor regulatory networks after retinal ganglion cell injury
TL;DR: In this article , the authors analyzed dysregulated lncRNAs associated with RGC injury, their potential regulatory functions, and the molecular mechanisms underlying the regulation of long noncoding RNAs and transcription factors (TFs).
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