Redefining the structural motifs that determine RNA binding and RNA editing by pentatricopeptide repeat proteins in land plants.
Shifeng Cheng,Bernard Gutmann,Xiao Zhong,Yongtao Ye,Mark F. Fisher,Fengqi Bai,Ian Castleden,Yue Song,Bo Song,Jiaying Huang,Xin Liu,Xun Xu,Boon Leong Lim,Charles S. Bond,Siu-Ming Yiu,Ian Small +15 more
TLDR
It is shown that the super-helical RNA-binding surface of RNA-editing factors is potentially longer than previously recognised, and used the redefined motifs to develop accurate and consistent annotations of PPR sequences from 109 genomes.Abstract:
The pentatricopeptide repeat (PPR) proteins form one of the largest protein families in land plants. They are characterised by tandem 30-40 amino acid motifs that form an extended binding surface capable of sequence-specific recognition of RNA strands. Almost all of them are post-translationally targeted to plastids and mitochondria, where they play important roles in post-transcriptional processes including splicing, RNA editing and the initiation of translation. A code describing how PPR proteins recognise their RNA targets promises to accelerate research on these proteins, but making use of this code requires accurate definition and annotation of all of the various nucleotide-binding motifs in each protein. We have used a structural modelling approach to define 10 different variants of the PPR motif found in plant proteins, in addition to the putative deaminase motif that is found at the C-terminus of many RNA-editing factors. We show that the super-helical RNA-binding surface of RNA-editing factors is potentially longer than previously recognised. We used the redefined motifs to develop accurate and consistent annotations of PPR sequences from 109 genomes. We report a high error rate in PPR gene models in many public plant proteomes, due to gene fusions and insertions of spurious introns. These consistently annotated datasets across a wide range of species are valuable resources for future comparative genomics studies, and an essential pre-requisite for accurate large-scale computational predictions of PPR targets. We have created a web portal (http://www.plantppr.com) that provides open access to these resources for the community.read more
Citations
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Shifting the limits in wheat research and breeding using a fully annotated reference genome
Rudi Appels,Rudi Appels,Kellye Eversole,Nils Stein,Nils Stein,Catherine Feuillet,Beat Keller,Jane Rogers,Curtis J. Pozniak,Frédéric Choulet,Assaf Distelfeld,Jesse Poland,Gil Ronen,Andrew G. Sharpe,Omer Barad,Kobi Baruch,Gabriel Keeble-Gagnère,Martin Mascher,Gil Ben-Zvi,Ambre-Aurore Josselin,Axel Himmelbach,François Balfourier,Juan J. Gutierrez-Gonzalez,Matthew J. Hayden,Chushin Koh,Gary J. Muehlbauer,Raj K. Pasam,Etienne Paux,Philippe Rigault,Josquin Tibbits,Vijay K. Tiwari,Manuel Spannagl,Daniel Lang,Heidrun Gundlach,Georg Haberer,Klaus F. X. Mayer,Danara Ormanbekova,Verena M. Prade,Hana Šimková,Thomas Wicker,David Swarbreck,Hélène Rimbert,Marius Felder,Nicolas Guilhot,Gemy Kaithakottil,Jens Keilwagen,Philippe Leroy,Thomas Lux,Sven Twardziok,Luca Venturini,Angéla Juhász,Michael Abrouk,Iris Fischer,Cristobal Uauy,Philippa Borrill,Ricardo H. Ramirez-Gonzalez,Dominique Arnaud,Smahane Chalabi,Boulos Chalhoub,Boulos Chalhoub,Aron T. Cory,Raju Datla,Mark W. Davey,John Jacobs,Stephen J. Robinson,Burkhard Steuernagel,Fred van Ex,Brande B. H. Wulff,Moussa Benhamed,Abdelhafid Bendahmane,Lorenzo Concia,David Latrasse,Jan Bartoš,Arnaud Bellec,Hélène Bergès,Jaroslav Doležel,Zeev Frenkel,Bikram S. Gill,Abraham B. Korol,Thomas Letellier,Odd-Arne Olsen,Kuldeep Singh,Miroslav Valárik,Edwin A. G. van der Vossen,Sonia Vautrin,Song Weining,Tzion Fahima,Vladimir Glikson,Dina Raats,Jarmila Číhalíková,Helena Toegelová,Jan Vrána,Pierre Sourdille,Benoit Darrier,D. Barabaschi,Luigi Cattivelli,Pilar Hernández,Sergio Gálvez,Hikmet Budak,Jonathan D. G. Jones,Kamil Witek,Guotai Yu,Ian Small,Joanna Melonek,Ruonan Zhou,Tatiana Belova,Kostya Kanyuka,Robert King,Kirby T. Nilsen,Sean Walkowiak,Richard D. Cuthbert,Ron Knox,Krysta Wiebe,Daoquan Xiang,Antje Rohde,Timothy Golds,Jana Čížková,Bala Ani Akpinar,Sezgi Biyiklioglu,Liangliang Gao,Amidou N’Daiye,Marie Kubaláková,Jan Šafář,Françoise Alfama,Anne-Françoise Adam-Blondon,Raphael Flores,Claire Guerche,Mikaël Loaec,Hadi Quesneville,Janet A. Condie,Jennifer Ens,Ron MacLachlan,Yifang Tan,Adriana Alberti,Jean-Marc Aury,Valérie Barbe,Arnaud Couloux,Corinne Cruaud,Karine Labadie,Sophie Mangenot,Patrick Wincker,Patrick Wincker,Gaganpreet Kaur,Ming-Cheng Luo,Sunish K. Sehgal,Parveen Chhuneja,O. P. Gupta,Suruchi Jindal,Parampreet Kaur,Palvi Malik,Priti Sharma,Bharat Yadav,Nagendra K. Singh,Jitendra P. Khurana,Chanderkant Chaudhary,Paramjit Khurana,Vinod Kumar,Ajay Kumar Mahato,Saloni Mathur,Amitha Mithra Sevanthi,Naveen Sharma,Ram Sewak Singh Tomar,Kateřina Holušová,Ondřej Plíhal,Matthew D. Clark,Matthew D. Clark,Darren Heavens,George Kettleborough,Jon Wright,Barbora Balcárková,Yuqin Hu,Elena A. Salina,Nikolai V. Ravin,Nikolai V. Ravin,Konstantin G. Skryabin,Konstantin G. Skryabin,Alexey V. Beletsky,Vitaly V. Kadnikov,Andrey V. Mardanov,Michail A. Nesterov,Andrey L. Rakitin,Ekaterina M. Sergeeva,Hirokazu Handa,Hiroyuki Kanamori,Satoshi Katagiri,Fuminori Kobayashi,Shuhei Nasuda,Tsuyoshi Tanaka,Jianzhong Wu,Federica Cattonaro,Min Jiumeng,Karl G. Kugler,Matthias Pfeifer,Simen Rød Sandve,Xu Xun,Bujie Zhan,Jacqueline Batley,Philipp E. Bayer,David Edwards,Satomi Hayashi,Zuzana Tulpová,Paul Visendi,Licao Cui,Xianghong Du,Kewei Feng,Xiaojun Nie,Wei Tong,Le Wang +207 more
TL;DR: This annotated reference sequence of wheat is a resource that can now drive disruptive innovation in wheat improvement, as this community resource establishes the foundation for accelerating wheat research and application through improved understanding of wheat biology and genomics-assisted breeding.
Journal ArticleDOI
Multiple wheat genomes reveal global variation in modern breeding.
Sean Walkowiak,Sean Walkowiak,Liangliang Gao,Cécile Monat,Georg Haberer,Mulualem T. Kassa,Jemima Brinton,Ricardo H. Ramirez-Gonzalez,Markus C. Kolodziej,Emily Delorean,Dinushika Thambugala,Valentyna Klymiuk,Brook Byrns,Heidrun Gundlach,Venkat Bandi,Jorge Nunez Siri,Kirby T. Nilsen,Catharine Aquino,Axel Himmelbach,Dario Copetti,Dario Copetti,Tomohiro Ban,Luca Venturini,Michael W. Bevan,Bernardo J. Clavijo,Dal-Hoe Koo,Jennifer Ens,Krystalee Wiebe,Amidou N’Diaye,Allen K. Fritz,Carl Gutwin,Anne Fiebig,Christine Fosker,Bin Xiao Fu,Gonzalo Garcia Accinelli,Keith A. Gardner,Nick Fradgley,Juan J. Gutierrez-Gonzalez,Gwyneth Halstead-Nussloch,Masaomi Hatakeyama,Chu Shin Koh,Jasline Deek,Alejandro C. Costamagna,Pierre R. Fobert,Darren Heavens,Hiroyuki Kanamori,Kanako Kawaura,Fuminori Kobayashi,Ksenia V. Krasileva,Tony Kuo,Tony Kuo,Neil McKenzie,Kazuki Murata,Yusuke Nabeka,Timothy Paape,Sudharsan Padmarasu,Lawrence Percival-Alwyn,Sateesh Kagale,Uwe Scholz,Jun Sese,Philomin Juliana,Ravi P. Singh,Rie Shimizu-Inatsugi,David Swarbreck,James Cockram,Hikmet Budak,Toshiaki Tameshige,Tsuyoshi Tanaka,Hiroyuki Tsuji,Jonathan M. Wright,Jianzhong Wu,Burkhard Steuernagel,Ian Small,Sylvie Cloutier,Gabriel Keeble-Gagnère,Gary J. Muehlbauer,Josquin Tibbets,Shuhei Nasuda,Joanna Melonek,Pierre Hucl,Andrew G. Sharpe,Matthew D. Clark,Erik Legg,Arvind K. Bharti,Peter Langridge,Anthony Hall,Cristobal Uauy,Martin Mascher,Simon G. Krattinger,Simon G. Krattinger,Hirokazu Handa,Kentaro Shimizu,Kentaro Shimizu,Assaf Distelfeld,Kenneth J. Chalmers,Beat Keller,Klaus F. X. Mayer,Jesse Poland,Nils Stein,Nils Stein,Curt A. McCartney,Manuel Spannagl,Thomas Wicker,Curtis J. Pozniak +103 more
TL;DR: Comparative analysis of multiple genome assemblies from wheat reveals extensive diversity that results from the complex breeding history of wheat and provides a basis for further potential improvements to this important food crop.
Journal ArticleDOI
Fern genomes elucidate land plant evolution and cyanobacterial symbioses
Fay-Wei Li,Fay-Wei Li,Paul Brouwer,Lorenzo Carretero-Paulet,Shifeng Cheng,Jan de Vries,Pierre-Marc Delaux,Ariana N. Eily,Nils Koppers,Li-Yaung Kuo,Zheng Li,Mathew Simenc,Ian Small,Eric K. Wafula,Stephany Angarita,Michael S. Barker,Andrea Bräutigam,Claude W. dePamphilis,Sven B. Gould,Prashant S. Hosmani,Yao Moan Huang,Bruno Huettel,Yoichiro Kato,Xin Liu,Steven Maere,Rose McDowell,Lukas A. Mueller,Klaas G.J. Nierop,Stefan A. Rensing,Tanner A. Robison,Carl J. Rothfels,Erin M. Sigel,Yue Song,Prakash R. Timilsena,Yves Van de Peer,Yves Van de Peer,Hongli Wang,Per K.I. Wilhelmsson,Paul G. Wolf,Xun Xu,Joshua P. Der,Henriette Schluepmann,Gane Ka-Shu Wong,Kathleen M. Pryer +43 more
TL;DR: The genomes of two fern species, Azolla filiculoides and Salvinia cucullata, are reported and insights into fern-specific whole-genome duplications, f Fern-specific insect-resistant gene evolution and fern–cyanobacterial symbiosis are provided.
Niche of harmful alga Aureococcus anophagefferens revealed through ecogenomics - eScholarship
TL;DR: A. anophagefferens possesses a larger genome (56 Mbp) and has more genes involved in light harvesting, organic carbon and nitrogen use, and encoding selenium- and metal-requiring enzymes than competing phytoplankton as mentioned in this paper.
Journal ArticleDOI
The hornwort genome and early land plant evolution
Jian Zhang,Xin Xing Fu,Rui Qi Li,Xiang Zhao,Yang Liu,Ming He Li,Arthur Zwaenepoel,Hong Ma,Bernard Goffinet,Yan Long Guan,Jia Yu Xue,Yi Ying Liao,Qing-Feng Wang,Qing‐Hua Wang,Jie Yu Wang,Jie Yu Wang,Guo-Qiang Zhang,Zhi Wen Wang,Yu Jia,Mei Zhi Wang,Shan-Shan Dong,Jian Fen Yang,Yuannian Jiao,Ya-Long Guo,Hongzhi Kong,An Ming Lu,Huanming Yang,Shou Zhou Zhang,Yves Van de Peer,Zhong-Jian Liu,Zhong-Jian Liu,Zhi-Duan Chen +31 more
TL;DR: A draft genome sequence of the hornwort Anthoceros augustus confirms the phylogenetic relationships among the three clades of bryophytes and provides insight into the unique characteristics of hornworts and their adaptations to live on land.
References
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TL;DR: The European Molecular Biology Open Software Suite is a mature package of software tools developed for the molecular biology community that includes a comprehensive set of applications for molecular sequence analysis and other tasks and integrates popular third-party software packages under a consistent interface.
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TL;DR: The nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome is determined using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map.
Book
Accelerated Profile HMM Searches
TL;DR: An acceleration heuristic for profile HMMs, the “multiple segment Viterbi” (MSV) algorithm, which computes an optimal sum of multiple ungapped local alignment segments using a striped vector-parallel approach previously described for fast Smith/Waterman alignment.
Journal ArticleDOI
Genome sequence of the human malaria parasite Plasmodium falciparum
Malcolm J. Gardner,Neil Hall,Eula Fung,Owen White,Matthew Berriman,Richard W. Hyman,Jane M. Carlton,Arnab Pain,Karen E. Nelson,Sharen Bowman,Ian T. Paulsen,Keith D. James,Jonathan A. Eisen,Kim Rutherford,Steven L. Salzberg,Alister Craig,Sue Kyes,Man Suen Chan,Vishvanath Nene,Shamira J. Shallom,Bernard B. Suh,Jeremy Peterson,Samuel V. Angiuoli,Mihaela Pertea,Jonathan E. Allen,Jeremy D. Selengut,Daniel H. Haft,Michael W. Mather,Akhil B. Vaidya,David M. A. Martin,Alan H. Fairlamb,Martin Fraunholz,David S. Roos,Stuart A. Ralph,Geoffrey I. McFadden,Leda M. Cummings,G. Mani Subramanian,Christopher J. Mungall,J. Craig Venter,Daniel J. Carucci,Stephen L. Hoffman,Chris I. Newbold,Ronald W. Davis,Claire M. Fraser,Bart Barrell +44 more
TL;DR: The genome sequence of P. falciparum clone 3D7 is reported, which is the most (A + T)-rich genome sequenced to date and is being exploited in the search for new drugs and vaccines to fight malaria.
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