Journal ArticleDOI
T-cell quality in memory and protection: implications for vaccine design
TLDR
The importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections is highlighted.Abstract:
T cells mediate effector functions through a variety of mechanisms. Recently, multiparameter flow cytometry has allowed a simultaneous assessment of the phenotype and multiple effector functions of single T cells; the delineation of T cells into distinct functional populations defines the quality of the response. New evidence suggests that the quality of T-cell responses is crucial for determining the disease outcome to various infections. This Review highlights the importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections.read more
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Cd123-specific chimeric antigen receptor redirected t cells and methods of their use
Stephen J. Forman,Armen Mardiros +1 more
TL;DR: A family of chimeric antigen receptors (CARs) containing an anti-CD123 scFv region fused in frame to a modified IgG4 hinge region comprising an S228P substitution, an L235E substitution, and optionally an N297Q substitution; a costimulatory signaling domain; and a T cell receptor (TCR) zeta chain signaling domain this article.
Journal ArticleDOI
Rapid, multiparameter profiling of cellular secretion using silicon photonic microring resonator arrays.
TL;DR: A silicon photonic biosensing chip capable of multiplexed protein measurements in a biomolecularly complex cell culture matrix is developed and the ability to discriminate between lineage commitments at early stages of culture differentiation is shown.
Journal ArticleDOI
T cell immunity to SARS-CoV-2 following natural infection and vaccination.
TL;DR: Some of the key mechanisms underlying cell-mediated immunity to respiratory viruses and the current understanding of the functional capacity and role of SARS-CoV-2-specific T cells following natural infection and vaccination are reviewed.
Journal ArticleDOI
Dendritic Cells Enhance Polyfunctionality of Adoptively Transferred T Cells That Target Cytomegalovirus in Glioblastoma.
Elizabeth A. Reap,Carter M. Suryadevara,Kristen A. Batich,Luis Sanchez-Perez,Gary E. Archer,Robert J. Schmittling,Pamela Norberg,James E. Herndon,Patrick Healy,Kendra L. Congdon,Patrick C. Gedeon,Olivia C. Campbell,Adam M. Swartz,Katherine A. Riccione,John S. Yi,Mohammed K. Hossain-Ibrahim,Anirudh Saraswathula,Smita K. Nair,Anastasie Dunn-Pirio,Taylor M. Broome,Kent J. Weinhold,Annick Desjardins,Gordana Vlahovic,Roger E. McLendon,Allan H. Friedman,Henry S. Friedman,Darell D. Bigner,Peter E. Fecci,Duane Mitchell,John H. Sampson +29 more
TL;DR: A randomized pilot trial in patients with newly diagnosed GBM implicates polyfunctional T-cell responses as a biomarker for effective antitumor immunotherapy and support a formal assessment of this combination approach in a larger randomized study.
Journal ArticleDOI
High Throughput T Epitope Mapping and Vaccine Development
TL;DR: The currently used methods are described with special reference to the possibility of applying the HTP concept to make epitope mapping an easier procedure in terms of time, workload, reagents, cells and overall cost.
References
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Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal ArticleDOI
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions
TL;DR: It is shown that expression of CCR7, a chemokine receptor that controls homing to secondary lymphoid organs, divides human memory T cells into two functionally distinct subsets, which are named central memory (TCM) and effector memory (TEM).
Journal ArticleDOI
Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8+ T Cells
Fiorenza Cocchi,Anthony L. DeVico,Alfredo Garzino-Demo,Suresh K. Arya,Robert C. Gallo,Paolo Lusso +5 more
TL;DR: Recombinant human RANTES, Mip-1α, and MIP-1β induced a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) and may have relevance for the prevention and therapy of AIDS.
Journal ArticleDOI
An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection.
JoAnne L. Flynn,John Chan,Karla J. Triebold,Dyana K. Dalton,Timothy A. Stewart,Barry R. Bloom +5 more
TL;DR: Gko mice have been developed which fail to produce IFN-gamma (gko), because of a targeted disruption of the gene for IFNs, and succumb to a rapid and fatal course of tuberculosis that could be delayed, but not prevented, by treatment with exogenous recombinant IFN.
Journal ArticleDOI
Subsets of human dendritic cell precursors express different toll-like receptors and respond to different microbial antigens.
Norimitsu Kadowaki,Stephen Ho,Svetlana Antonenko,Rene de Waal Malefyt,Robert A. Kastelein,J. Fernando Bazan,Yong-Jun Liu +6 more
TL;DR: The expression of distinct sets of TLRs and the corresponding difference in reactivity to microbial molecules among subsets of pre-DCs and imDCs support the concept that they have developed through distinct evolutionary pathways to recognize different microbial antigens.