Journal ArticleDOI
T-cell quality in memory and protection: implications for vaccine design
TLDR
The importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections is highlighted.Abstract:
T cells mediate effector functions through a variety of mechanisms. Recently, multiparameter flow cytometry has allowed a simultaneous assessment of the phenotype and multiple effector functions of single T cells; the delineation of T cells into distinct functional populations defines the quality of the response. New evidence suggests that the quality of T-cell responses is crucial for determining the disease outcome to various infections. This Review highlights the importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections.read more
Citations
More filters
Journal ArticleDOI
Cutaneous Leishmaniasis Vaccination: A Matter of Quality
TL;DR: Evaluating the quality of immune response against Leishmania, using a multiparametric analysis in order to understand better this complex host-parasite interaction, and discussing the differences in the responses triggered by different New World Leishmaniasis species, as well as the impact on the development of an effective vaccine against CL.
Journal ArticleDOI
Age Distribution of Multiple Functionally Relevant Subsets of CD4+ T Cells in Human Blood Using a Standardized and Validated 14-Color EuroFlow Immune Monitoring Tube.
Vitor Botafogo,Martin Perez-Andres,Martin Perez-Andres,María Jara-Acevedo,María Jara-Acevedo,Paloma Bárcena,Paloma Bárcena,Georgiana Grigore,Alejandro Hernández-Delgado,Daniela Damasceno,Daniela Damasceno,Suzanne Comans,Elena Blanco,Elena Blanco,Alfonso Romero,Sonia Arriba-Méndez,Irene Gastaca-Abasolo,Carlos E. Pedreira,Jacqueline A. M. van Gaans-van den Brink,Véronique Corbière,Françoise Mascart,Cécile A. C. M. van Els,Alex-Mikael Barkoff,Andrea Mayado,Andrea Mayado,Jacques J.M. van Dongen,Julia Almeida,Julia Almeida,Alberto Orfao,Alberto Orfao +29 more
TL;DR: The EuroFlow immune monitoring TCD4 tube allows fast, automated, and reproducible identification of ≥89 subsets of CD4+ blood T cells, with different kinetics throughout life, and set the basis for in-depth T-cell monitoring in different disease and therapeutic conditions.
Journal ArticleDOI
Customized Viral Immunotherapy for HPV-Associated Cancer
Matthew J. Atherton,Kyle B Stephenson,Jonathan Pol,Fuan Wang,Charles Lefebvre,David F. Stojdl,Jake K Nikota,Anna Dvorkin-Gheva,Andrew Nguyen,Lan Chen,Stephanie Johnson-Obaseki,Patrick James Villeneuve,Jean-Simon Diallo,Jim Dimitroulakos,Yonghong Wan,Brian D. Lichty +15 more
TL;DR: MG1-Maraba can acutely alter the tumor microenvironment in vivo and exploit molecular hallmarks of HPV-associated cancer, as demonstrated by marked infection of HPV+ patient tumor biopsies and is ideally suited as an oncolytic treatment against clinical HPV+ cancer.
Journal ArticleDOI
Single Cell Functional Proteomics for Assessing Immune Response in Cancer Therapy: Technology, Methods, and Applications
Chao Ma,Rong Fan,Meltem Elitas +2 more
TL;DR: New technologies have been developed to directly profile the cellular immune functions and the functional heterogeneity and will likely contribute to new understanding of therapy mechanism, pre-treatment stratification of patients, and ongoing therapeutic monitoring and assessment.
Journal ArticleDOI
Evaluation of the safety and immunogenicity in rhesus monkeys of a recombinant malaria vaccine for Plasmodium vivax with a synthetic Toll-like receptor 4 agonist formulated in an emulsion.
Joanne M. Lumsden,Sathit Pichyangkul,Utaiwan Srichairatanakul,Kosol Yongvanitchit,Amporn Limsalakpetch,Saule Nurmukhambetova,Jennifer H. Klein,Sylvie Bertholet,Thomas S. Vedvick,Steven G. Reed,Jetsumon Sattabongkot,Jason W. Bennett,Mark E. Polhemus,Christian F. Ockenhouse,Randall F. Howard,Anjali Yadava +15 more
TL;DR: The combination of VMP001 and GLA-SE is safe and immunogenic in monkeys and may serve as a potential second-generation vaccine candidate against P. vivax malaria.
References
More filters
Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal ArticleDOI
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions
TL;DR: It is shown that expression of CCR7, a chemokine receptor that controls homing to secondary lymphoid organs, divides human memory T cells into two functionally distinct subsets, which are named central memory (TCM) and effector memory (TEM).
Journal ArticleDOI
Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8+ T Cells
Fiorenza Cocchi,Anthony L. DeVico,Alfredo Garzino-Demo,Suresh K. Arya,Robert C. Gallo,Paolo Lusso +5 more
TL;DR: Recombinant human RANTES, Mip-1α, and MIP-1β induced a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) and may have relevance for the prevention and therapy of AIDS.
Journal ArticleDOI
An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection.
JoAnne L. Flynn,John Chan,Karla J. Triebold,Dyana K. Dalton,Timothy A. Stewart,Barry R. Bloom +5 more
TL;DR: Gko mice have been developed which fail to produce IFN-gamma (gko), because of a targeted disruption of the gene for IFNs, and succumb to a rapid and fatal course of tuberculosis that could be delayed, but not prevented, by treatment with exogenous recombinant IFN.
Journal ArticleDOI
Subsets of human dendritic cell precursors express different toll-like receptors and respond to different microbial antigens.
Norimitsu Kadowaki,Stephen Ho,Svetlana Antonenko,Rene de Waal Malefyt,Robert A. Kastelein,J. Fernando Bazan,Yong-Jun Liu +6 more
TL;DR: The expression of distinct sets of TLRs and the corresponding difference in reactivity to microbial molecules among subsets of pre-DCs and imDCs support the concept that they have developed through distinct evolutionary pathways to recognize different microbial antigens.