Journal ArticleDOI
T-cell quality in memory and protection: implications for vaccine design
TLDR
The importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections is highlighted.Abstract:
T cells mediate effector functions through a variety of mechanisms. Recently, multiparameter flow cytometry has allowed a simultaneous assessment of the phenotype and multiple effector functions of single T cells; the delineation of T cells into distinct functional populations defines the quality of the response. New evidence suggests that the quality of T-cell responses is crucial for determining the disease outcome to various infections. This Review highlights the importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections.read more
Citations
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A high-throughput single-cell analysis of human CD8 + T cell functions reveals discordance for cytokine secretion and cytolysis
Navin Varadarajan,Boris Julg,Yvonne J. Yamanaka,Huabiao Chen,Adebola O. Ogunniyi,Elizabeth G. McAndrew,Lindsay C. Porter,Alicja Piechocka-Trocha,Brenna J. Hill,Daniel C. Douek,Florencia Pereyra,Bruce D. Walker,J. Christopher Love +12 more
TL;DR: A high-throughput, automated assay in 125-pl microwells is developed to simultaneously evaluate the ability of thousands of individual CD8+ T cells from HIV-infected patients to mediate lysis and to produce cytokines, and investigate the correlation between immediate cytotoxic activity and short-term cytokine secretion.
Journal ArticleDOI
The role of mTOR in memory CD8 T-cell differentiation.
TL;DR: The role of mTOR in memory CD8+ T‐cell differentiation is reviewed and the effect of rapamycin among CD8 + T cells, CD4+ T cells and dendritic cells is compared to discuss potential application of these findings in a clinical setting.
Journal ArticleDOI
Control of chronic mycobacterium tuberculosis infection by CD4 KLRG1- IL-2-secreting central memory cells
TL;DR: Results suggest that the CD4+ KLRG1− IL-2–secreting subsets are central memory T cells with the potential to continuously replenish the T cells at the site of infection and prevent attrition and functional exhaustion.
Journal ArticleDOI
Antigens reversibly conjugated to a polymeric glyco-adjuvant induce protective humoral and cellular immunity
D. Scott Wilson,D. Scott Wilson,Sachiko Hirosue,Michal M. Raczy,Michal M. Raczy,Leonardo Bonilla-Ramirez,Laura Jeanbart,Ruyi Wang,Marcin Kwissa,Jean-François Franetich,Maria A. S. Broggi,Maria A. S. Broggi,Giacomo Diaceri,Xavier Quaglia-Thermes,Dominique Mazier,Melody A. Swartz,Melody A. Swartz,Jeffrey A. Hubbell,Jeffrey A. Hubbell +18 more
TL;DR: The antigen–p(Man–TLR7) platform offers a strategy to enhance the immunogenicity of protein subunit vaccines and generates potent cellular and humoral immune response against malaria.
Journal ArticleDOI
Cellular and molecular synergy in AS01-adjuvanted vaccines results in an early IFNγ response promoting vaccine immunogenicity.
Margherita Coccia,Catherine Collignon,Caroline Hervé,Aurélie Chalon,Iain Welsby,Sophie Detienne,Mary J. van Helden,Sheetij Dutta,Christopher J. Genito,Norman C. Waters,Katrijn Van Deun,Katrijn Van Deun,Age K. Smilde,Robert A. van den Berg,David Franco,Patricia Bourguignon,Sandra Morel,Nathalie Garçon,Bart N. Lambrecht,Stanislas Goriely,Robbert van der Most,Arnaud M. Didierlaurent +21 more
TL;DR: In this article, the authors reported a unique mechanism of molecular and cellular synergy between a TLR4 ligand, 3-Odesacyl-4'-monophosphoryl lipid A (MPL), and a saponin, QS-21, the constituents of the Adjuvant System AS01.
References
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Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal ArticleDOI
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions
TL;DR: It is shown that expression of CCR7, a chemokine receptor that controls homing to secondary lymphoid organs, divides human memory T cells into two functionally distinct subsets, which are named central memory (TCM) and effector memory (TEM).
Journal ArticleDOI
Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8+ T Cells
Fiorenza Cocchi,Anthony L. DeVico,Alfredo Garzino-Demo,Suresh K. Arya,Robert C. Gallo,Paolo Lusso +5 more
TL;DR: Recombinant human RANTES, Mip-1α, and MIP-1β induced a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) and may have relevance for the prevention and therapy of AIDS.
Journal ArticleDOI
An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection.
JoAnne L. Flynn,John Chan,Karla J. Triebold,Dyana K. Dalton,Timothy A. Stewart,Barry R. Bloom +5 more
TL;DR: Gko mice have been developed which fail to produce IFN-gamma (gko), because of a targeted disruption of the gene for IFNs, and succumb to a rapid and fatal course of tuberculosis that could be delayed, but not prevented, by treatment with exogenous recombinant IFN.
Journal ArticleDOI
Subsets of human dendritic cell precursors express different toll-like receptors and respond to different microbial antigens.
Norimitsu Kadowaki,Stephen Ho,Svetlana Antonenko,Rene de Waal Malefyt,Robert A. Kastelein,J. Fernando Bazan,Yong-Jun Liu +6 more
TL;DR: The expression of distinct sets of TLRs and the corresponding difference in reactivity to microbial molecules among subsets of pre-DCs and imDCs support the concept that they have developed through distinct evolutionary pathways to recognize different microbial antigens.