Journal ArticleDOI
T-cell quality in memory and protection: implications for vaccine design
TLDR
The importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections is highlighted.Abstract:
T cells mediate effector functions through a variety of mechanisms. Recently, multiparameter flow cytometry has allowed a simultaneous assessment of the phenotype and multiple effector functions of single T cells; the delineation of T cells into distinct functional populations defines the quality of the response. New evidence suggests that the quality of T-cell responses is crucial for determining the disease outcome to various infections. This Review highlights the importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections.read more
Citations
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Pitfalls of vaccinations with WT1-, Proteinase3- and MUC1-derived peptides in combination with MontanideISA51 and CpG7909.
Jürgen Kuball,Karin de Boer,Eva Wagner,Mohammed Wattad,Edite Antunes,Risini D. Weeratna,Alain Vicari,Alain Vicari,Carina Lotz,Suzanne van Dorp,Samantha Hol,Philip D. Greenberg,Philip D. Greenberg,Wolfgang Heit,Heather L. Davis,Matthias Theobald +15 more
TL;DR: To test safety and immunogenicity of a vaccine composed of WT1, Pr3, and MUC1-derived Class I-restricted peptides and the pan HLA-DR T helper cell epitope (PADRE), four patients with AML and five with MM were repetitively vaccinated with MontanideISA51 and CpG7909.
Journal ArticleDOI
Regulatory T cells suppress antiviral immune responses and increase viral loads during acute infection with a lymphotropic retrovirus.
TL;DR: The authors showed that depletion of Tregs amplified the immune responses in draining lymph nodes at the site of infection, but at the same time delayed the entry of the immune cells into the HSV-2-infected tissue.
Journal ArticleDOI
BK polyomavirus-specific cellular immune responses are age-dependent and strongly correlate with phases of virus replication.
Tina Schmidt,C. Adam,Hans H. Hirsch,Hans H. Hirsch,Martin Janssen,M. Wolf,Jan Dirks,Piotr Kardas,Piotr Kardas,Thurid Ahlenstiel-Grunow,Lars Pape,Tilman R. Rohrer,Danilo Fliser,Martina Sester,Urban Sester +14 more
TL;DR: BKPyV‐specific cellular immunity reflects phases of active BK PyV‐replication either after primary infection in childhood or during reactivation after transplantation, and combined analysis of BKPyv‐specific T cell functionality and viral loads may improve individual risk assessment.
Journal ArticleDOI
The early cellular signatures of protective immunity induced by live viral vaccination
Siegfried Kohler,Nicole Bethke,Matthias Böthe,Sophie Sommerick,Marco Frentsch,Chiara Romagnani,Matthias Niedrig,Andreas Thiel +7 more
TL;DR: Interestingly, potent adaptive immunity as measured by high titers of neutralizing YFV‐17D‐specific antibodies, correlated with early activation and recruitment of YFv‐17d‐specific CD4+ T cells and higher levels of sIL‐6R.
Journal ArticleDOI
Visualizing the Functional Diversification of CD8+ T Cell Responses in Lymph Nodes
Hélène Beuneu,Hélène Beuneu,Fabrice Lemaître,Fabrice Lemaître,Jacques Deguine,Jacques Deguine,Hélène D. Moreau,Hélène D. Moreau,Isabelle Bouvier,Isabelle Bouvier,Zacarias Garcia,Zacarias Garcia,Matthew L. Albert,Matthew L. Albert,Philippe Bousso,Philippe Bousso +15 more
TL;DR: Imaged T cells expressing a fluorescent reporter for the activation of the interferon-γ (IFN-γ) locus during priming in lymph nodes and demonstrated marked differences in the efficiency of gene activation during stable T cell-dentritic cell (DC) contacts, influenced in part by signal strength.
References
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Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal ArticleDOI
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions
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Journal ArticleDOI
Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8+ T Cells
Fiorenza Cocchi,Anthony L. DeVico,Alfredo Garzino-Demo,Suresh K. Arya,Robert C. Gallo,Paolo Lusso +5 more
TL;DR: Recombinant human RANTES, Mip-1α, and MIP-1β induced a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) and may have relevance for the prevention and therapy of AIDS.
Journal ArticleDOI
An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection.
JoAnne L. Flynn,John Chan,Karla J. Triebold,Dyana K. Dalton,Timothy A. Stewart,Barry R. Bloom +5 more
TL;DR: Gko mice have been developed which fail to produce IFN-gamma (gko), because of a targeted disruption of the gene for IFNs, and succumb to a rapid and fatal course of tuberculosis that could be delayed, but not prevented, by treatment with exogenous recombinant IFN.
Journal ArticleDOI
Subsets of human dendritic cell precursors express different toll-like receptors and respond to different microbial antigens.
Norimitsu Kadowaki,Stephen Ho,Svetlana Antonenko,Rene de Waal Malefyt,Robert A. Kastelein,J. Fernando Bazan,Yong-Jun Liu +6 more
TL;DR: The expression of distinct sets of TLRs and the corresponding difference in reactivity to microbial molecules among subsets of pre-DCs and imDCs support the concept that they have developed through distinct evolutionary pathways to recognize different microbial antigens.