Journal ArticleDOI
T-cell quality in memory and protection: implications for vaccine design
TLDR
The importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections is highlighted.Abstract:
T cells mediate effector functions through a variety of mechanisms. Recently, multiparameter flow cytometry has allowed a simultaneous assessment of the phenotype and multiple effector functions of single T cells; the delineation of T cells into distinct functional populations defines the quality of the response. New evidence suggests that the quality of T-cell responses is crucial for determining the disease outcome to various infections. This Review highlights the importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections.read more
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High-Throughput Secretomic Analysis of Single Cells to Assess Functional Cellular Heterogeneity
Yao Lu,Jonathan J. Chen,Luye Mu,Qiong Xue,Yu Wu,Pei Hsun Wu,Jie Li,Alexander O. Vortmeyer,Kathryn Miller-Jensen,Denis Wirtz,Rong Fan +10 more
TL;DR: A simple bioanalytical assay platform consisting of a large array of subnanoliter microchambers integrated with high-density antibody barcode microarrays for highly multiplexed protein detection from over a thousand single cells in parallel is described, observing distinct heterogeneity among the single cell secretomic signatures that can be directly correlated to the cells' physical behavior such as migration.
Journal ArticleDOI
Rv3615c is a highly immunodominant RD1 (Region of Difference 1)-dependent secreted antigen specific for Mycobacterium tuberculosis infection
Kerry A. Millington,Sarah M. Fortune,Jeffrey B. Low,Alejandra Garces,Suzanne M. Hingley-Wilson,Melissa Wickremasinghe,Onn Min Kon,Ajit Lalvani +7 more
TL;DR: The high immunodominance of EspC, equivalent to that of ESAT-6 and CFP-10, makes it a TB vaccine candidate, and its high specificity confers strong potential for T-cell–based immunodiagnosis.
Journal ArticleDOI
Pro- and anti-inflammatory cytokines in cutaneous leishmaniasis: a review.
TL;DR: The role of pro- and anti-inflammatory cytokines in the immunoprotection and immunopathology of CL is reviewed.
Journal ArticleDOI
Immune markers and correlates of protection for vaccine induced immune responses
TL;DR: Different immune markers and new candidates for correlates of a protective vaccine induced immune response against chronic infections are reviewed and how successful they have been in defining the protective immunity in human and veterinary medicine.
Journal ArticleDOI
Impact of donor CMV status on viral infection and reconstitution of multifunction CMV-specific T cells in CMV-positive transplant recipients
Wendi Zhou,Jeff Longmate,Simon F. Lacey,Joycelynne M. Palmer,Ghislaine Gallez-Hawkins,Lia Thao,Ricardo Spielberger,Ryotaro Nakamura,Stephen J. Forman,John A. Zaia,Don J. Diamond +10 more
TL;DR: The results highlight the benefit of D(+) donors in improving outcomes of R(+) HCT recipients by reducing the duration and recurrent need of antiviral treatment, aided by increased levels of multifunctional CMV-specific T cells.
References
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Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
Journal ArticleDOI
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions
TL;DR: It is shown that expression of CCR7, a chemokine receptor that controls homing to secondary lymphoid organs, divides human memory T cells into two functionally distinct subsets, which are named central memory (TCM) and effector memory (TEM).
Journal ArticleDOI
Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8+ T Cells
Fiorenza Cocchi,Anthony L. DeVico,Alfredo Garzino-Demo,Suresh K. Arya,Robert C. Gallo,Paolo Lusso +5 more
TL;DR: Recombinant human RANTES, Mip-1α, and MIP-1β induced a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) and may have relevance for the prevention and therapy of AIDS.
Journal ArticleDOI
An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection.
JoAnne L. Flynn,John Chan,Karla J. Triebold,Dyana K. Dalton,Timothy A. Stewart,Barry R. Bloom +5 more
TL;DR: Gko mice have been developed which fail to produce IFN-gamma (gko), because of a targeted disruption of the gene for IFNs, and succumb to a rapid and fatal course of tuberculosis that could be delayed, but not prevented, by treatment with exogenous recombinant IFN.
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Subsets of human dendritic cell precursors express different toll-like receptors and respond to different microbial antigens.
Norimitsu Kadowaki,Stephen Ho,Svetlana Antonenko,Rene de Waal Malefyt,Robert A. Kastelein,J. Fernando Bazan,Yong-Jun Liu +6 more
TL;DR: The expression of distinct sets of TLRs and the corresponding difference in reactivity to microbial molecules among subsets of pre-DCs and imDCs support the concept that they have developed through distinct evolutionary pathways to recognize different microbial antigens.