Journal ArticleDOI
T-cell quality in memory and protection: implications for vaccine design
TLDR
The importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections is highlighted.Abstract:
T cells mediate effector functions through a variety of mechanisms. Recently, multiparameter flow cytometry has allowed a simultaneous assessment of the phenotype and multiple effector functions of single T cells; the delineation of T cells into distinct functional populations defines the quality of the response. New evidence suggests that the quality of T-cell responses is crucial for determining the disease outcome to various infections. This Review highlights the importance of using multiparameter flow cytometry to better understand the functional capacity of effector and memory T-cell responses, thereby enabling the development of preventative and therapeutic vaccine strategies for infections.read more
Citations
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Journal ArticleDOI
Narrowing of T-cell receptor beta variable repertoire during symptomatic herpesvirus infection in transplant patients.
Katherine K Wynn,Katherine K Wynn,Tania Crough,Scott B. Campbell,Keith McNeil,Andrew J. Galbraith,Denis J. Moss,Sharon L. Silins,Scott C. Bell,Rajiv Khanna +9 more
TL;DR: It is shown that SOT recipients who maintain peripheral blood TRBV repertoire diversity and functional antigen‐specific T‐cell responses can resist clinical symptomatic disease in spite of high levels of viral load.
Journal ArticleDOI
The Role of Lymphatic Niches in T Cell Differentiation
Tara Capece,Minsoo Kim +1 more
TL;DR: This review aims to highlight the cellularity of various niches within the lymph node and emphasize recent evidence suggesting that distinct types of T cell activation and differentiation occur within different immune contexts in lymphoid organs.
Journal ArticleDOI
Label-Free Imaging of Single Proteins Secreted from Living Cells via ISCAT Microscopy
André Gemeinhardt,Matthew P. McDonald,Katharina König,Michael Aigner,Andreas Mackensen,Vahid Sandoghdar,Vahid Sandoghdar +6 more
TL;DR: In this protocol, the fundamental steps to realize an iSCAT microscope are covered and the method for real-time detection of single proteins as they are secreted from a living cell which is demonstrated with an immortalized B-cell line.
Journal ArticleDOI
High throughput cellular screens to interrogate the human T and B cell repertoires
TL;DR: These culture-based methods can be used to interrogate with high efficiency the immune repertoires in order to characterize the frequency, function and specificity of naïve or memory T cells and to isolate potent and broadly neutralizing monoclonal antibodies.
Journal ArticleDOI
CD40 ligand enhances immunogenicity of vector-based vaccines in immunocompetent and CD4+ T cell deficient individuals
TL;DR: Findings show that CD40L has the capacity to enhance the magnitude of vaccine-induced polyfunctional T cell responses in CD4+ T cell deficient mice, and warrants further study as an adjuvant for immunization against opportunistic pathogens in individuals with CD4-ablated animals.
References
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Journal Article
Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.
TL;DR: A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished.
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Two subsets of memory T lymphocytes with distinct homing potentials and effector functions
TL;DR: It is shown that expression of CCR7, a chemokine receptor that controls homing to secondary lymphoid organs, divides human memory T cells into two functionally distinct subsets, which are named central memory (TCM) and effector memory (TEM).
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Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8+ T Cells
Fiorenza Cocchi,Anthony L. DeVico,Alfredo Garzino-Demo,Suresh K. Arya,Robert C. Gallo,Paolo Lusso +5 more
TL;DR: Recombinant human RANTES, Mip-1α, and MIP-1β induced a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) and may have relevance for the prevention and therapy of AIDS.
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An essential role for interferon gamma in resistance to Mycobacterium tuberculosis infection.
JoAnne L. Flynn,John Chan,Karla J. Triebold,Dyana K. Dalton,Timothy A. Stewart,Barry R. Bloom +5 more
TL;DR: Gko mice have been developed which fail to produce IFN-gamma (gko), because of a targeted disruption of the gene for IFNs, and succumb to a rapid and fatal course of tuberculosis that could be delayed, but not prevented, by treatment with exogenous recombinant IFN.
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Subsets of human dendritic cell precursors express different toll-like receptors and respond to different microbial antigens.
Norimitsu Kadowaki,Stephen Ho,Svetlana Antonenko,Rene de Waal Malefyt,Robert A. Kastelein,J. Fernando Bazan,Yong-Jun Liu +6 more
TL;DR: The expression of distinct sets of TLRs and the corresponding difference in reactivity to microbial molecules among subsets of pre-DCs and imDCs support the concept that they have developed through distinct evolutionary pathways to recognize different microbial antigens.