Dramatic rise in plasma viremia after CD8+ T cell depletion in simian immunodeficiency virus-infected macaques
Xia Jin,Daniel E. Bauer,Sarah Tuttleton,Sharon R Lewin,Agegnehu Gettie,James Blanchard,Craig E. Irwin,Jeffrey T. Safrit,John E. Mittler,Leor S. Weinberger,Leondios G. Kostrikis,Linqi Zhang,Alan S. Perelson,David D. Ho +13 more
TLDR
It is demonstrated that CD8 cells play a crucial role in suppressing SIV replication in vivo and are examined using an anti-CD8 monoclonal antibody, OKT8F.Abstract:
To determine the role of CD8(+) T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction of CD8 cells in peripheral blood in six infected Macaca mulatta treated with OKT8F. The apparent CD8 depletion started 1 h after antibody administration, and low CD8 levels were maintained until day 8. An increase in plasma viremia of one to three orders of magnitude was observed in five of the six macaques. The injection of a control antibody to an infected macaque did not induce a sustained viral load increase, nor did it significantly reduce the number of CD8(+) T cells. These results demonstrate that CD8 cells play a crucial role in suppressing SIV replication in vivo.read more
Citations
More filters
Journal ArticleDOI
The impact of highly active antiretroviral therapy on HIV-specific immune function.
TL;DR: There has been movement toward combining HAART with various means of augmenting and/or reconstituting the host's immune system, especially HIV-1-specific immune responses, especially the long-term goal is to discontinue HAART and permit the reconstitute immune system to contain whatever small amounts of the virus remain.
Journal ArticleDOI
Evaluation of combination DNA/replication-competent Ad-SIV recombinant immunization regimens in rhesus macaques.
Nina Malkevitch,Daniela Rohne,Joel Pinczewski,Kristine Aldrich,Vaniambadi S. Kalyanaraman,Norman L. Letvin,Marjorie Robert-Guroff +6 more
TL;DR: It is concluded that two sequential immunizations with the same replication-competent Ad5hr-SIV recombinant is as effective as priming with one or two recombinant DNA vaccines followed by a single Ad5HRS IV recombinant immunization.
Journal ArticleDOI
Evaluation of CD8+ T-cell and antibody responses following transient increased viraemia in rhesus macaques infected with live, attenuated simian immunodeficiency virus.
Karin J. Metzner,Walter J. Moretto,Sean M. Donahoe,Xia Jin,Agegnehu Gettie,David C. Montefiori,Preston A. Marx,James M. Binley,Douglas F. Nixon,Ruth I. Connor +9 more
TL;DR: It is suggested that a transient period of enhanced antigenaemia during chronic SIV infection may serve to augment virus-specific immunity in some, but not all, macaques.
Journal ArticleDOI
Protection from Secondary Human Immunodeficiency Virus Type 1 Infection in Chimpanzees Suggests the Importance of Antigenic Boosting and a Possible Role for Cytotoxic T Cells
Sunita S. Balla-Jhagjhoorsingh,Petra Mooij,P. J. F. Ten Haaft,Willy M. J. M. Bogers,Vera J. P. Teeuwsen,Gerrit Koopman,Jonathan L. Heeney +6 more
TL;DR: It is suggested that immunologic boosting by HIV-1 antigens or exposure to subinfectious doses of virus may be important events in sustaining sufficient immunity to prevent secondary infections from occurring.
Journal ArticleDOI
CD8 T cells targeting adapted epitopes in chronic HIV infection promote dendritic cell maturation and CD4 T cell trans-infection.
Kai Qin,Sushma Boppana,Victor Y. Du,Jonathan M. Carlson,Ling Yue,Dario A. Dilernia,Eric Hunter,Robbie B. Mailliard,Simon Mallal,Anju Bansal,Paul A. Goepfert +10 more
TL;DR: The data suggests that the emergence of AE-specific CD8 T cell responses in CHI confers a selective advantage to the virus by promoting DC-mediated CD4 T cell trans-infection.
References
More filters
Journal ArticleDOI
Molecular Beacons: Probes that Fluoresce upon Hybridization
Sanjay Tyagi,Fred Russell Kramer +1 more
TL;DR: Novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions that undergo a spontaneous conforma-tional change when they hybridize to their targets are developed.
Journal Article
Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67.
TL;DR: The data suggest that the early stages of mitogen stimulation represent initial sequences of proliferation and not parts of the cell cycle, and immunostaining with monoclonal antibody Ki-67 provides a reliable means of rapidly evaluating the growth fraction of normal and neoplastic human cell populations.
Journal ArticleDOI
HIV-1 Dynamics in Vivo: Virion Clearance Rate, Infected Cell Life-Span, and Viral Generation Time
TL;DR: A new mathematical model was used to analyze a detailed set of human immunodeficiency virus-type 1 (HIV-1) viral load data collected from five infected individuals after the administration of a potent inhibitor of HIV-1 protease, providing not only a kinetic picture ofAIDS pathogenesis, but also theoretical principles to guide the development of treatment strategies.
Journal ArticleDOI
Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8+ T Cells
Fiorenza Cocchi,Anthony L. DeVico,Alfredo Garzino-Demo,Suresh K. Arya,Robert C. Gallo,Paolo Lusso +5 more
TL;DR: Recombinant human RANTES, Mip-1α, and MIP-1β induced a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) and may have relevance for the prevention and therapy of AIDS.
Journal ArticleDOI
Prognosis in HIV-1 Infection Predicted by the Quantity of Virus in Plasma
John W. Mellors,Charles R. Rinaldo,Phalguni Gupta,Roseanne M. White,John Todd,Lawrence A. Kingsley +5 more
TL;DR: Plasma viral load was a better predictor of progression to AIDS and death than was the number of CD4+ T cells, and the risk of acquired immunodeficiency syndrome (AIDS) and death in study subjects was directly related to plasma viral load at study entry.