Dramatic rise in plasma viremia after CD8+ T cell depletion in simian immunodeficiency virus-infected macaques
Xia Jin,Daniel E. Bauer,Sarah Tuttleton,Sharon R Lewin,Agegnehu Gettie,James Blanchard,Craig E. Irwin,Jeffrey T. Safrit,John E. Mittler,Leor S. Weinberger,Leondios G. Kostrikis,Linqi Zhang,Alan S. Perelson,David D. Ho +13 more
TLDR
It is demonstrated that CD8 cells play a crucial role in suppressing SIV replication in vivo and are examined using an anti-CD8 monoclonal antibody, OKT8F.Abstract:
To determine the role of CD8(+) T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction of CD8 cells in peripheral blood in six infected Macaca mulatta treated with OKT8F. The apparent CD8 depletion started 1 h after antibody administration, and low CD8 levels were maintained until day 8. An increase in plasma viremia of one to three orders of magnitude was observed in five of the six macaques. The injection of a control antibody to an infected macaque did not induce a sustained viral load increase, nor did it significantly reduce the number of CD8(+) T cells. These results demonstrate that CD8 cells play a crucial role in suppressing SIV replication in vivo.read more
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Presentation of SIVgag to monkey T cells using dendritic cells transfected with a recombinant adenovirus
Lei Zhong,Angela Granelli-Piperno,Melissa Pope,Ralf Ignatius,Mark G. Lewis,Sarah Schlesinger Frankel,Sarah Schlesinger Frankel,Ralph M. Steinman +7 more
TL;DR: Recombinant adenoviruses efficiently transfect monkey DC in a nonperturbing fashion, and these DC efficiently present antigens to SIVgag immune CD8+ T cells, which will allow autologous DC, expressing SIV genes with high efficiency, to be tested in vivo to achieve strong specific T cell immunity.
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The HIV-1 Antisense Protein (ASP) induces CD8 T cell responses during chronic infection
Anne Bet,Emmanuel Atangana Maze,Emmanuel Atangana Maze,Emmanuel Atangana Maze,Anju Bansal,Sarah Sterrett,Antoine Gross,Stéphanie Graff-Dubois,Stéphanie Graff-Dubois,Stéphanie Graff-Dubois,Assia Samri,Assia Samri,Amélie Guihot,Amélie Guihot,Christine Katlama,Christine Katlama,Ioannis Theodorou,Ioannis Theodorou,Jean-Michel Mesnard,Arnaud Moris,Paul A. Goepfert,Sylvain Cardinaud +21 more
TL;DR: The results provide the first description of CD8+ T cell-mediated immune responses to ASP in HIV-1-infected patients, demonstrating that ASP is expressed during infection.
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Killing Kinetics of Simian Immunodeficiency Virus-Specific CD8+ T Cells: Implications for HIV Vaccine Strategies
Erik Rollman,Miranda Zoe Denham Smith,Andrew G. Brooks,Damian F. J. Purcell,Bartek Zuber,Ian A. Ramshaw,Stephen J. Kent +6 more
TL;DR: The delayed cytolytic phenotype exhibited by standard vector-based vaccine-induced CTLs may limit the ability of T cell-based HIV vaccines to rapidly control acute infection following a pathogenic lentiviral exposure.
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Prevention of influenza virus shedding and protection from lethal H1N1 challenge using a consensus 2009 H1N1 HA and NA adenovirus vector vaccine.
Frank R. Jones,Elizabeth Gabitzsch,Younong Xu,Joseph P. Balint,Viktoriya Borisevich,Jennifer K. Smith,Jeanon N. Smith,Bi Hung Peng,Aida G. Walker,Magda Salazar,Slobodan Paessler +10 more
TL;DR: Vaccines against emerging pathogens such as the 2009 H1N1 pandemic virus can benefit from current technologies such as rapid genomic sequencing to construct the most biologically relevant vaccine.
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Efficacy of Multivalent Adenovirus-Based Vaccine against Simian Immunodeficiency Virus Challenge
Danilo R. Casimiro,Kara S. Cox,Aimin Tang,Kara J. Sykes,Meizhen Feng,Fubao Wang,Andrew J. Bett,William A. Schleif,Xiaoping Liang,Jessica A. Flynn,Timothy W. Tobery,Keith A. Wilson,Adam C. Finnefrock,Lingyi Huang,Salvatore Vitelli,Jing Lin,Deepa Patel,Mary Ellen Davies,Gwendolyn J. Heidecker,Daniel C. Freed,Sheri Dubey,David H. O’Connor,David I. Watkins,Zhiqiang Zhang,John W. Shiver +24 more
TL;DR: Examination of prophylactic efficacies of several multivalent replication-incompetent adenovirus serotype 5 (Ad5) vaccines in rhesus macaques found expansion of the antigen breadth resulted in more favorable virological outcomes.
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