Dramatic rise in plasma viremia after CD8+ T cell depletion in simian immunodeficiency virus-infected macaques
Xia Jin,Daniel E. Bauer,Sarah Tuttleton,Sharon R Lewin,Agegnehu Gettie,James Blanchard,Craig E. Irwin,Jeffrey T. Safrit,John E. Mittler,Leor S. Weinberger,Leondios G. Kostrikis,Linqi Zhang,Alan S. Perelson,David D. Ho +13 more
TLDR
It is demonstrated that CD8 cells play a crucial role in suppressing SIV replication in vivo and are examined using an anti-CD8 monoclonal antibody, OKT8F.Abstract:
To determine the role of CD8(+) T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction of CD8 cells in peripheral blood in six infected Macaca mulatta treated with OKT8F. The apparent CD8 depletion started 1 h after antibody administration, and low CD8 levels were maintained until day 8. An increase in plasma viremia of one to three orders of magnitude was observed in five of the six macaques. The injection of a control antibody to an infected macaque did not induce a sustained viral load increase, nor did it significantly reduce the number of CD8(+) T cells. These results demonstrate that CD8 cells play a crucial role in suppressing SIV replication in vivo.read more
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In-vitro viral suppressive capacity correlates with immune checkpoint marker expression on peripheral CD8+ T cells in treated HIV-positive patients.
Pieter Pannus,Pieter Pannus,Pieter Pannus,Philipp Adams,Philipp Adams,Elisabeth Willems,Leo Heyndrickx,Eric Florence,Sofie Rutsaert,Ward De Spiegelaere,Linos Vandekerckhove,Carole Seguin-Devaux,Guido Vanham,Guido Vanham +13 more
TL;DR: It is found that the capacity of CD8+ T cells to suppress viral replication is increased after stimulation with HIV-1 peptides, and this VSC was correlated with expression of immune checkpoint markers, which are generally considered to be markers of exhaustion.
Book ChapterDOI
Monkey Models and HIV Vaccine Research.
TL;DR: This chapter summarizes major scientific evidence generated in these models since the beginning of the AIDS pandemic and hopes the accumulated knowledge and lessons contributed by thousands of scientists will be useful in promoting the search of an ultimate solution to end HIV/AIDS.
Journal ArticleDOI
In vivo depletion of CD8+ cells does not affect primary maedi visna virus infection in sheep.
TL;DR: Surprisingly, these animals displayed a normal induction of pCTL whereas the virus-specific proliferative responses were reduced, which could reflect either that a proportion of functional CD8+ lymphocytes remained in these animals, as suggested by the appearance of p CTLs, or that CD8- cells are not required for control of primary MVV infection.
BookDOI
Control of innate and adaptive immune responses during infectious diseases
TL;DR: The editor of this volume, having research interests in the field of ROS production and the damage to cellular systems, has identified a number of enzymes showing ·OH scavenging activities details of which are anticipated to be published in the near future.
Journal ArticleDOI
A structural constraint for functional interaction between N-terminal and C-terminal domains in simian immunodeficiency virus capsid proteins.
Natsuko F. Inagaki,Hiroaki Takeuchi,Masaru Yokoyama,Hironori Sato,Akihide Ryo,Hiroyuki Yamamoto,Miki Kawada,Tetsuro Matano +7 more
TL;DR: In vitro and in vivo evidence is presented implicating the interaction between Gag residues 205 in CA NTD and 340 in CA CTD in SIV replication, providing insight into immunogen design to limit viral escape options.
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