Dramatic rise in plasma viremia after CD8+ T cell depletion in simian immunodeficiency virus-infected macaques
Xia Jin,Daniel E. Bauer,Sarah Tuttleton,Sharon R Lewin,Agegnehu Gettie,James Blanchard,Craig E. Irwin,Jeffrey T. Safrit,John E. Mittler,Leor S. Weinberger,Leondios G. Kostrikis,Linqi Zhang,Alan S. Perelson,David D. Ho +13 more
TLDR
It is demonstrated that CD8 cells play a crucial role in suppressing SIV replication in vivo and are examined using an anti-CD8 monoclonal antibody, OKT8F.Abstract:
To determine the role of CD8(+) T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction of CD8 cells in peripheral blood in six infected Macaca mulatta treated with OKT8F. The apparent CD8 depletion started 1 h after antibody administration, and low CD8 levels were maintained until day 8. An increase in plasma viremia of one to three orders of magnitude was observed in five of the six macaques. The injection of a control antibody to an infected macaque did not induce a sustained viral load increase, nor did it significantly reduce the number of CD8(+) T cells. These results demonstrate that CD8 cells play a crucial role in suppressing SIV replication in vivo.read more
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Mucosal priming with PEI/DNA complex and systemic boosting with recombinant TianTan vaccinia stimulate vigorous mucosal and systemic immune responses.
Xianggang Huang,Jianqing Xu,Chao Qiu,Li Ren,Lianxing Liu,Yanmin Wan,Ning Zhang,Hong Peng,Yiming Shao +8 more
TL;DR: The results demonstrate that the combination of intranasal priming with PEI/DNA complexes and systemic boosting with rTTV is a preferable regimen for induction of both T-cell and humoral immune responses.
Patent
Immunomodulating compositions, uses therefor and processes for their production
TL;DR: The use of at least one set of peptides in compositions and methods for modulating an immune response to one or more polypeptide antigens is discussed in this article.
Journal ArticleDOI
Primary replication of a recombinant Sendai virus vector in macaques.
Munehide Kano,Tetsuro Matano,Tetsuro Matano,Atsushi Kato,Hiromi Nakamura,Akiko Takeda,Yuriko Suzaki,Yasushi Ami,Keiji Terao,Yoshiyuki Nagai +9 more
TL;DR: Gag expression was undetectable in the lung as well as in remote lymphoid tissues, such as the thymus, spleen and inguinal LN, indicating that the spread of the virus was more restricted in macaques than in mice.
Journal ArticleDOI
Comparison of T cell immune responses induced by vectored HIV vaccines in non-human primates and humans.
Andrew J. Bett,Sheri Dubey,Devan V. Mehrotra,Liming Guan,Romnie Long,Kiersten Anderson,Kelly B. Collins,Christine Gaunt,Rose Fernandez,Suzanne Cole,Steve Meschino,Aimin Tang,Xiao Sun,Sanjay Gurunathan,Jim Tartaglia,Michael N. Robertson,John W. Shiver,Danilo R. Casimiro +17 more
TL;DR: Although the immunogenicity of the vaccines and vaccine regimens evaluated were not all accurately predicted, testing in rhesus macaques generally offers an indispensable tool for ranking the immunological potential of HIV-1 vaccine candidates.
Journal ArticleDOI
Evaluation of the immunogenicity of replication-competent V-knocked-out and replication-defective F-deleted Sendai virus vector-based vaccines in macaques.
Akiko Takeda,Hiroko Igarashi,Miki Kawada,Tetsuo Tsukamoto,Hiroyuki Yamamoto,Makoto Inoue,Akihiro Iida,Tsugumine Shu,Mamoru Hasegawa,Tetsuro Matano,Tetsuro Matano +10 more
TL;DR: The immunogenicity of replication-competent V-knocked-out and replication-defective F-deleted SeV vectors in macaques was evaluated to show the potential of recombinant Sendai virus (SeV) vectors to induce virus-specific T-cell responses in macaque AIDS models.
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