Dramatic rise in plasma viremia after CD8+ T cell depletion in simian immunodeficiency virus-infected macaques
Xia Jin,Daniel E. Bauer,Sarah Tuttleton,Sharon R Lewin,Agegnehu Gettie,James Blanchard,Craig E. Irwin,Jeffrey T. Safrit,John E. Mittler,Leor S. Weinberger,Leondios G. Kostrikis,Linqi Zhang,Alan S. Perelson,David D. Ho +13 more
TLDR
It is demonstrated that CD8 cells play a crucial role in suppressing SIV replication in vivo and are examined using an anti-CD8 monoclonal antibody, OKT8F.Abstract:
To determine the role of CD8(+) T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction of CD8 cells in peripheral blood in six infected Macaca mulatta treated with OKT8F. The apparent CD8 depletion started 1 h after antibody administration, and low CD8 levels were maintained until day 8. An increase in plasma viremia of one to three orders of magnitude was observed in five of the six macaques. The injection of a control antibody to an infected macaque did not induce a sustained viral load increase, nor did it significantly reduce the number of CD8(+) T cells. These results demonstrate that CD8 cells play a crucial role in suppressing SIV replication in vivo.read more
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Comprehensive screening reveals strong and broadly directed human immunodeficiency virus type 1-specific CD8 responses in perinatally infected children.
Margaret E. Feeney,Kathleen A. Roosevelt,Yanhua Tang,Katja Pfafferott,Kenneth McIntosh,Sandra K. Burchett,Charlotte Mao,Bruce D. Walker,Philip J. R. Goulder,Philip J. R. Goulder +9 more
TL;DR: This data indicate that perinatally infected children who survive infancy mount a robust HIV-1-specific CD8 response that is much stronger than previously thought and is comparable in magnitude and breadth to that of adults.
Journal ArticleDOI
Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous simian immunodeficiency virus-specific CD8+ T cell clones in rhesus macaques during acute infection.
Jacob T. Minang,Matthew T. Trivett,Diane L. Bolton,Charles M. Trubey,Jacob D. Estes,Yuan Li,Jeremy Smedley,Rhonda Pung,Margherita Rosati,Rashmi Jalah,George N. Pavlakis,Barbara K. Felber,Michael Piatak,Mario Roederer,Jeffrey D. Lifson,David E. Ott,Claes Ohlen +16 more
TL;DR: These studies establish methods for adoptive transfer of autologous SIV-specific CD8+ T cells for evaluating immune control during acute infection and demonstrate that infused cells retain function and persist for at least 2 mo in specific tissues.
Journal ArticleDOI
Vaccination with Attenuated Simian Immunodeficiency Virus by DNA Inoculation
TL;DR: Delivering attenuated lentivirus vaccines as proviral DNA would be simple and inexpensive and provide substantial protection against subsequent mucosal SIVmac251 challenge.
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Impact of the genetic restriction of virus-specific T-cell responses in hepatitis C virus infection
C Neumann-Haefelin,R Thimme +1 more
TL;DR: The outcome and course of HCV infection is most likely determined by a complex interplay of genetic, immunological and virological factors, and a better understanding of these host–virus interactions is essential for the development of new antiviral vaccine strategies.
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Controlled release of PEI/DNA complexes from PLGA microspheres as a potent delivery system to enhance immune response to HIV vaccine DNA prime/MVA boost regime.
Xianfeng Zhou,Bin Liu,Xianghui Yu,Xiao Zha,Xizhen Zhang,Xueyun Wang,Yinghua Jin,Yongge Wu,Yue Chen,Yaming Shan,Yan Chen,Junqiu Liu,Wei Kong,Jiacong Shen +13 more
TL;DR: A novel approach involving the preparation of biodegradable PLGA microspheres containing entrapping complexes of DNA and polyethylenimine was developed to improve the delivery of DNA into antigen-presenting cells after intramuscular injection and induced significantly enhanced serum antibody responses 2-3 orders of magnitude greater than naked DNA.
References
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Journal ArticleDOI
Molecular Beacons: Probes that Fluoresce upon Hybridization
Sanjay Tyagi,Fred Russell Kramer +1 more
TL;DR: Novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions that undergo a spontaneous conforma-tional change when they hybridize to their targets are developed.
Journal Article
Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67.
TL;DR: The data suggest that the early stages of mitogen stimulation represent initial sequences of proliferation and not parts of the cell cycle, and immunostaining with monoclonal antibody Ki-67 provides a reliable means of rapidly evaluating the growth fraction of normal and neoplastic human cell populations.
Journal ArticleDOI
HIV-1 Dynamics in Vivo: Virion Clearance Rate, Infected Cell Life-Span, and Viral Generation Time
TL;DR: A new mathematical model was used to analyze a detailed set of human immunodeficiency virus-type 1 (HIV-1) viral load data collected from five infected individuals after the administration of a potent inhibitor of HIV-1 protease, providing not only a kinetic picture ofAIDS pathogenesis, but also theoretical principles to guide the development of treatment strategies.
Journal ArticleDOI
Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8+ T Cells
Fiorenza Cocchi,Anthony L. DeVico,Alfredo Garzino-Demo,Suresh K. Arya,Robert C. Gallo,Paolo Lusso +5 more
TL;DR: Recombinant human RANTES, Mip-1α, and MIP-1β induced a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) and may have relevance for the prevention and therapy of AIDS.
Journal ArticleDOI
Prognosis in HIV-1 Infection Predicted by the Quantity of Virus in Plasma
John W. Mellors,Charles R. Rinaldo,Phalguni Gupta,Roseanne M. White,John Todd,Lawrence A. Kingsley +5 more
TL;DR: Plasma viral load was a better predictor of progression to AIDS and death than was the number of CD4+ T cells, and the risk of acquired immunodeficiency syndrome (AIDS) and death in study subjects was directly related to plasma viral load at study entry.