Dramatic rise in plasma viremia after CD8+ T cell depletion in simian immunodeficiency virus-infected macaques
Xia Jin,Daniel E. Bauer,Sarah Tuttleton,Sharon R Lewin,Agegnehu Gettie,James Blanchard,Craig E. Irwin,Jeffrey T. Safrit,John E. Mittler,Leor S. Weinberger,Leondios G. Kostrikis,Linqi Zhang,Alan S. Perelson,David D. Ho +13 more
TLDR
It is demonstrated that CD8 cells play a crucial role in suppressing SIV replication in vivo and are examined using an anti-CD8 monoclonal antibody, OKT8F.Abstract:
To determine the role of CD8(+) T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction of CD8 cells in peripheral blood in six infected Macaca mulatta treated with OKT8F. The apparent CD8 depletion started 1 h after antibody administration, and low CD8 levels were maintained until day 8. An increase in plasma viremia of one to three orders of magnitude was observed in five of the six macaques. The injection of a control antibody to an infected macaque did not induce a sustained viral load increase, nor did it significantly reduce the number of CD8(+) T cells. These results demonstrate that CD8 cells play a crucial role in suppressing SIV replication in vivo.read more
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Plasmacytoid dendritic cell and functional HIV Gag p55-specific T cells before treatment interruption can inform set-point plasma HIV viral load after treatment interruption in chronically suppressed HIV-1(+) patients.
Emmanouil Papasavvas,Andrea S. Foulkes,Xiangfan Yin,Jocelin Joseph,Brian N Ross,Livio Azzoni,Jay R. Kostman,Karam Mounzer,Jane Shull,Luis J. Montaner +9 more
TL;DR: The dual contribution of pDC and anti‐HIV T‐cell responses to viral control suggests that these variables may serve as immune correlates of viral control and could be integrated in cure or ART‐intensification strategies.
Journal ArticleDOI
Heterogeneity of the simian immunodeficiency virus (SIV) specific CD8(+) T-cell response in mucosal tissues during SIV primary infection.
TL;DR: The frequencies of SIV-specific gamma-IFN-secreting CD8(+) T cells are low in the mucosa during early primary infection, which may be of importance with regard to the intense viral replication observed inThe mucosa at this stage.
Journal ArticleDOI
Vaccine-induced T cells Provide Partial Protection Against High-dose Rectal SIVmac239 Challenge of Rhesus Macaques
Marcio O. Lasaro,Larissa H. Haut,Larissa H. Haut,Xiangyang Zhou,Zhiquan Xiang,Dongming Zhou,Yan Li,Wynetta Giles-Davis,Hua Li,Jessica C. Engram,Lauren J. DiMenna,Ang Bian,Marina Sazanovich,Elizabeth Parzych,Raj K. Kurupati,Juliana C. Small,Te Lang Wu,R.M. Leskowitz,Nicole R. Klatt,Jason M. Brenchley,David A. Garber,Mark G. Lewis,Sarah J. Ratcliffe,Michael R. Betts,Guido Silvestri,Guido Silvestri,Hildegund C. J. Ertl +26 more
TL;DR: It is suggested that T cells, while unable to affect SIV acquisition upon high-dose rectal infection, can reduce disease progression and induction of potent T-cell responses should remain a component of the authors' efforts to develop an efficacious vaccine to HIV-1.
Journal ArticleDOI
Independence of Granzyme B Secretion and Interferon-γ Production during Acute Simian Immunodeficiency Virus Infection
Sandra A. Calarota,Miguel Otero,Tara M. Robinson,Anlan Dai,Mark G. Lewis,Jean D. Boyer,David B. Weiner +6 more
TL;DR: The data support the concept that the GrB and IFN-gamma ELISPOT assays measure immune responses in different immune-cell populations with unique specificities.
Journal ArticleDOI
Differential patterns of immune escape at Tat-specific cytotoxic T cell epitopes in pigtail macaques.
TL;DR: This work studied CTL responses to regulatory and accessory proteins of SIV in pigtail macaques to suggest that some regulatory or accessory CTL epitopes may be useful targets for vaccination against HIV.
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