Dramatic rise in plasma viremia after CD8+ T cell depletion in simian immunodeficiency virus-infected macaques
Xia Jin,Daniel E. Bauer,Sarah Tuttleton,Sharon R Lewin,Agegnehu Gettie,James Blanchard,Craig E. Irwin,Jeffrey T. Safrit,John E. Mittler,Leor S. Weinberger,Leondios G. Kostrikis,Linqi Zhang,Alan S. Perelson,David D. Ho +13 more
TLDR
It is demonstrated that CD8 cells play a crucial role in suppressing SIV replication in vivo and are examined using an anti-CD8 monoclonal antibody, OKT8F.Abstract:
To determine the role of CD8(+) T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction of CD8 cells in peripheral blood in six infected Macaca mulatta treated with OKT8F. The apparent CD8 depletion started 1 h after antibody administration, and low CD8 levels were maintained until day 8. An increase in plasma viremia of one to three orders of magnitude was observed in five of the six macaques. The injection of a control antibody to an infected macaque did not induce a sustained viral load increase, nor did it significantly reduce the number of CD8(+) T cells. These results demonstrate that CD8 cells play a crucial role in suppressing SIV replication in vivo.read more
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Concordant proficiency in measurement of T-cell immunity in human immunodeficiency virus vaccine clinical trials by peripheral blood mononuclear cell and enzyme-linked immunospot assays in laboratories from three continents
Mark Boaz,Peter Hayes,Tony Tarragona,Laura Seamons,Andrew Cooper,Josephine Birungi,Paul Kato Kitandwe,Aloysius Semaganda,Pontiano Kaleebu,Gwynneth Stevens,Omu Anzala,Bashir Farah,Simon Ogola,Jackton Indangasi,Patrick Mhlanga,Melanie Van Eeden,Madhuri Thakar,Ashwini Pujari,Shadri Mishra,Nilu Goonetilleke,Stephen Moore,Abdul Mahmoud,Pattabiraman Sathyamoorthy,Jayashri Mahalingam,P. R. Narayanan,V. D. Ramanathan,Josephine H. Cox,Len Dally,Dilbinder K. Gill,Jill Gilmour +29 more
TL;DR: The results of these proficiency panels demonstrate the ability of seven laboratories, located across three continents, to process PBMC samples and to rank volunteers with differential magnitudes of IFN-γ ELISPOT responses and illustrate the ability to standardize the IFn-γ ElisPOT assay across multiple laboratories when common training methods, reagents such as fetal calf serum, and standard operating procedures are adopted.
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An Adjuvanted Polyprotein HIV-1 Vaccine Induces Polyfunctional Cross-Reactive CD4+ T Cell Responses in Seronegative Volunteers
Eva Van Braeckel,Patricia Bourguignon,Marguerite Koutsoukos,Frédéric Clement,Michel Janssens,Isabelle Carletti,Alix Collard,Marie-Ange Demoitié,Gerald Voss,Geert Leroux-Roels,Lisa McNally +10 more
TL;DR: A novel AS01-adjuvanted HIV-1 vaccine candidate consisting of a recombinant fusion protein (F4) containing 4 HIV- 1 clade B antigens induced long-lasting, polyfunctional cross-reactive CD4+ T-cell responses in HIV-seronegative volunteers.
Journal ArticleDOI
Superior control of HIV-1 replication by CD8+ T cells targeting conserved epitopes: implications for HIV vaccine design.
Pratima Kunwar,Natalie Hawkins,Warren L. Dinges,Yi Liu,Erin E. Gabriel,David A. Swan,Claire E. Stevens,Janine Maenza,Ann C. Collier,James I. Mullins,Tomer Hertz,Xuesong Yu,Helen Horton,Helen Horton +13 more
TL;DR: The next-generation of T-cell based HIV-1 vaccines should focus on strategies that can elicit CD8+ T cell responses to multiple conserved epitopes of HIV- said to be independent of HLA type.
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Construction and immunogenicity in a prime-boost regimen of a Semliki Forest virus-vectored experimental HIV clade A vaccine.
Tomáš Hanke,Christina Barnfield,Edmund G.-T. Wee,Lena Ågren,Rachel V. Samuel,Natasha Larke,Peter Liljeström +6 more
TL;DR: A novel, experimental subunit human immunodeficiency virus (HIV) vaccine, SFV.HIVA, was constructed and was shown to be as effective in T cell induction as pTHr and less immunogenic than MVA.
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Immune response to HIV.
TL;DR: The kinetics of the immunological and virological events occurring during primary HIV infection indicate that the establishment of the latent HIV reservoir likely occurs during the very early stages of primary infection, prior to the development of the HIV-specific immune response which has limited efficacy in the control of the early events of infection.
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Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8+ T Cells
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