Dramatic rise in plasma viremia after CD8+ T cell depletion in simian immunodeficiency virus-infected macaques
Xia Jin,Daniel E. Bauer,Sarah Tuttleton,Sharon R Lewin,Agegnehu Gettie,James Blanchard,Craig E. Irwin,Jeffrey T. Safrit,John E. Mittler,Leor S. Weinberger,Leondios G. Kostrikis,Linqi Zhang,Alan S. Perelson,David D. Ho +13 more
TLDR
It is demonstrated that CD8 cells play a crucial role in suppressing SIV replication in vivo and are examined using an anti-CD8 monoclonal antibody, OKT8F.Abstract:
To determine the role of CD8(+) T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction of CD8 cells in peripheral blood in six infected Macaca mulatta treated with OKT8F. The apparent CD8 depletion started 1 h after antibody administration, and low CD8 levels were maintained until day 8. An increase in plasma viremia of one to three orders of magnitude was observed in five of the six macaques. The injection of a control antibody to an infected macaque did not induce a sustained viral load increase, nor did it significantly reduce the number of CD8(+) T cells. These results demonstrate that CD8 cells play a crucial role in suppressing SIV replication in vivo.read more
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Virus-Specific Cellular Immune Correlates of Survival in Vaccinated Monkeys after Simian Immunodeficiency Virus Challenge
Yue Sun,Jörn E. Schmitz,Adam P. Buzby,Brianne R. Barker,Srinivas S. Rao,Ling Xu,Zhi Yong Yang,John R. Mascola,Gary J. Nabel,Norman L. Letvin,Norman L. Letvin +10 more
TL;DR: It is demonstrated that SIV-specific IFN-γ, tumor necrosis factor alpha-, and interleukin-2-producing T lymphocytes are all comparably associated with protection against disease progression, underscore the contribution of virus-specific central memory T lymphocyte to controlling clinical progression in vaccinated individuals following a primate immunodeficiency virus infection.
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Relative Dominance of Epitope-Specific Cytotoxic T-Lymphocyte Responses in Human Immunodeficiency Virus Type 1-Infected Persons with Shared HLA Alleles
Cheryl L. Day,Amy K. Shea,Marcus Altfeld,Douglas P. Olson,Susan Buchbinder,Frederick Hecht,Eric S. Rosenberg,Bruce D. Walker,Spyros A. Kalams +8 more
TL;DR: Analysis of the CTL response to optimally defined CTL epitopes restricted by HLA class I A and B alleles in individuals who coexpressed HLA A2, A3, and B7 indicates that HLA type alone does not predict CTL responses and that numerous potential epitopes may not be targeted by CTL in a given individual.
Journal Article
CD8 T-cell responses in early HIV-1 infection are skewed towards high entropy peptides.
Anju Bansal,Ethan K. Gough,Steffanie Sabbaj,Doug Ritter,Karina Yusim,Greg Sfakianos,Grace M. Aldrovandi,Richard A. Kaslow,Craig M. Wilson,Mark J. Mulligan,J. Michael Kilby,Paul A. Goepfert +11 more
TL;DR: It is demonstrated that HIV-specific CD8 T cells are directed preferentially to the variable peptides in early infection but diminish in frequency during chronic disease, in large part due to cytotoxic T lymphocyte escape.
Journal ArticleDOI
Dynamics of T-Cell Responses and Memory T Cells during Primary Simian Immunodeficiency Virus Infection in Cynomolgus Macaques
Ingrid Karlsson,Benoit Malleret,Patricia Brochard,Benoit Delache,Julien Calvo,Roger Le Grand,Roger Le Grand,Bruno Vaslin,Bruno Vaslin +8 more
TL;DR: An inverse relationship between plasma viral load and the simian immunodeficiency virus (SIV)-specific T cells responses in peripheral blood and lymph nodes is found and details about the changes of virus-specific immune responses over time are provided.
Journal ArticleDOI
Induction of Potent Human Immunodeficiency Virus Type 1-Specific T-Cell-Restricted Immunity by Genetically Modified Dendritic Cells
Julianna Lisziewicz,Dmitry I. Gabrilovich,Georg Varga,Jianqing Xu,Philip D. Greenberg,Suresh K. Arya,Marnix L. Bosch,Jean-Paul Behr,Franco Lori +8 more
TL;DR: A novel technology combining replication- and integration-defective human immunodeficiency virus type 1 (HIV-1) vectors with genetically modified dendritic cells was developed in order to induce T-cell immunity, circumventing the problem of obtaining viral vector expression in the absence of integration.
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