Dramatic rise in plasma viremia after CD8+ T cell depletion in simian immunodeficiency virus-infected macaques
Xia Jin,Daniel E. Bauer,Sarah Tuttleton,Sharon R Lewin,Agegnehu Gettie,James Blanchard,Craig E. Irwin,Jeffrey T. Safrit,John E. Mittler,Leor S. Weinberger,Leondios G. Kostrikis,Linqi Zhang,Alan S. Perelson,David D. Ho +13 more
TLDR
It is demonstrated that CD8 cells play a crucial role in suppressing SIV replication in vivo and are examined using an anti-CD8 monoclonal antibody, OKT8F.Abstract:
To determine the role of CD8(+) T cells in controlling simian immunodeficiency virus (SIV) replication in vivo, we examined the effect of depleting this cell population using an anti-CD8 monoclonal antibody, OKT8F. There was on average a 99.9% reduction of CD8 cells in peripheral blood in six infected Macaca mulatta treated with OKT8F. The apparent CD8 depletion started 1 h after antibody administration, and low CD8 levels were maintained until day 8. An increase in plasma viremia of one to three orders of magnitude was observed in five of the six macaques. The injection of a control antibody to an infected macaque did not induce a sustained viral load increase, nor did it significantly reduce the number of CD8(+) T cells. These results demonstrate that CD8 cells play a crucial role in suppressing SIV replication in vivo.read more
Citations
More filters
Journal ArticleDOI
Distribution and functional analysis of memory antiviral CD8 T cell responses in HIV-1 and cytomegalovirus infections.
Kim Ellefsen,Alexandre Harari,Patrick Champagne,Pierre-Alexandre Bart,Rafick-Pierre Sekaly,Giuseppe Pantaleo +5 more
TL;DR: In this article, the anatomic distribution and the function of different populations of HIV-1 and cytomegalovirus (CMV)-specific memory CD8 T cells were investigated in subjects with chronic HIV 1 and CMV co-infection.
Journal ArticleDOI
Potentiation of Simian Immunodeficiency Virus (SIV)-Specific CD4+ and CD8+ T Cell Responses by a DNA-SIV and NYVAC-SIV Prime/Boost Regimen
Zdeněk Hel,Wen-Po Tsai,Arthur Thornton,Janos Nacsa,Laura Giuliani,Elzbieta Tryniszewska,Monita Poudyal,David Venzon,Xiaochi Wang,John D. Altman,David I. Watkins,Wenhong Lu,Agneta von Gegerfelt,Barbara K. Felber,James Tartaglia,George N. Pavlakis,Genoveffa Franchini +16 more
TL;DR: In macaques immunized with the prime-boost regimen, the extent and durability of CD8+ T cell response to an immunodominant SIV gag epitope was increased and these animals recognized a broader array of subdominant SIV epitopes in the cytolytic assay.
Patent
Packaging of immunostimulatory oligonucleotides into virus-like particles: method of preparation and use
Martin F. Bachmann,Andreas Cornelius,Vania Manolova,Patrik Maurer,Edwin Meijerink,Karl G. Proba,Katrin Schwarz +6 more
TL;DR: In this paper, it was shown that DNA oligonucleotides containing non-methylated C and G (CpGs) can be loaded with immunostimulatory substances and induce enhanced B and T cell responses.
Journal ArticleDOI
Cytotoxic T lymphocyte responses to human immunodeficiency virus: control and escape.
TL;DR: The mechanisms through which HIV evades CTL activity are outlined, and compelling evidence that cytotoxic T lymphocytes constitute an essential component of protective antiretroviral immunity is provided.
Journal ArticleDOI
Control of Simian/Human Immunodeficiency Virus Viremia and Disease Progression after IL-2-Augmented DNA-Modified Vaccinia Virus Ankara Nasal Vaccination in Nonhuman Primates
Frederic M.N. Bertley,Pamela A. Kozlowski,Pamela A. Kozlowski,Shainn Wei Wang,Shainn Wei Wang,Joseph Chappelle,Jignesh Patel,Oluwakemi Sonuyi,Gail P. Mazzara,David C. Montefiori,Angela Carville,Keith G. Mansfield,Anna Aldovini +12 more
TL;DR: Results of a combined DNA-modified vaccinia virus Ankara (MVA) vaccine approach that stimulated simian/human immunodeficiency virus (SHIV)-specific immune responses by vaccination at the nasal mucosa indicate that nasal vaccination with SHIV-DNA plus IL-2/Ig DNA and rMVA can provide significant protection from disease progression.
References
More filters
Journal ArticleDOI
Molecular Beacons: Probes that Fluoresce upon Hybridization
Sanjay Tyagi,Fred Russell Kramer +1 more
TL;DR: Novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions that undergo a spontaneous conforma-tional change when they hybridize to their targets are developed.
Journal Article
Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67.
TL;DR: The data suggest that the early stages of mitogen stimulation represent initial sequences of proliferation and not parts of the cell cycle, and immunostaining with monoclonal antibody Ki-67 provides a reliable means of rapidly evaluating the growth fraction of normal and neoplastic human cell populations.
Journal ArticleDOI
HIV-1 Dynamics in Vivo: Virion Clearance Rate, Infected Cell Life-Span, and Viral Generation Time
TL;DR: A new mathematical model was used to analyze a detailed set of human immunodeficiency virus-type 1 (HIV-1) viral load data collected from five infected individuals after the administration of a potent inhibitor of HIV-1 protease, providing not only a kinetic picture ofAIDS pathogenesis, but also theoretical principles to guide the development of treatment strategies.
Journal ArticleDOI
Identification of RANTES, MIP-1α, and MIP-1β as the Major HIV-Suppressive Factors Produced by CD8+ T Cells
Fiorenza Cocchi,Anthony L. DeVico,Alfredo Garzino-Demo,Suresh K. Arya,Robert C. Gallo,Paolo Lusso +5 more
TL;DR: Recombinant human RANTES, Mip-1α, and MIP-1β induced a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) and may have relevance for the prevention and therapy of AIDS.
Journal ArticleDOI
Prognosis in HIV-1 Infection Predicted by the Quantity of Virus in Plasma
John W. Mellors,Charles R. Rinaldo,Phalguni Gupta,Roseanne M. White,John Todd,Lawrence A. Kingsley +5 more
TL;DR: Plasma viral load was a better predictor of progression to AIDS and death than was the number of CD4+ T cells, and the risk of acquired immunodeficiency syndrome (AIDS) and death in study subjects was directly related to plasma viral load at study entry.