Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Journal ArticleDOI
The Rotation of Mouse Myoblast Nuclei is Dependent on Substrate Elasticity
Ryan J. Hickey,Andrew E. Pelling +1 more
TL;DR: This work studied nuclear rotation in mouse C2C12 myoblasts cultured on soft substrates designed to mimic resting tissue and on hard glass substrates and demonstrated the clear dependence of nuclear rotation dynamics on matrix stiffness.
Book ChapterDOI
On the Application of Multiphasic Theories to the Problem of Cellsubstrate Mechanical Interactions
TL;DR: After discussing the general formalism based on balance laws and constitutive relation, the chapter introduces a numerical strategy, based on the extended finite element, to capture the contraction of cells on compliant substrate.
Book ChapterDOI
Finite Element Quantification of the Compressive Forces Induced by Keratinocyte on a Liquid Crystal Substrate
Chin Fhong Soon,Chin Fhong Soon,Mansour Youseffi,Peter C. Twigg,Nicholas Blagden,Morgan Denyer +5 more
TL;DR: In this paper, a method of transducing keratinocyte traction forces in the form of compression on the surface of a cholesteryl ester liquid crystal substrate has been developed.
Journal ArticleDOI
Cell polarization energy and its implications for cell migration
TL;DR: An energy model is proposed to study the cell polarization energy by considering the effect of matrix rigidity, cell shape, and organization of the cytoskeleton and it is demonstrated that the fibroblast-like cell shape and keratocyte-like shape both have an optimal polarization angle corresponding to the most stable cell shape.
Dissertation
Formulation of aqueous core capsules for the triggered release of proteins
TL;DR: The first temperature-sensitive capsules able to release their content upon increasing the temperature above a threshold are obtained, representing a promising route to the biocompatible delivery of proteins.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
Journal ArticleDOI
Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
Journal ArticleDOI
Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.