Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Journal ArticleDOI
Human mammary epithelial cells in a mature, stratified epithelial layer flatten and stiffen compared to single and confluent cells
TL;DR: In this article, a combination of atomic force, fluorescence and confocal microscopy was used to determine the changes in stiffness, morphology and actin distribution of human mammary epithelial cells as they transition from single cells to confluency to a mature layer.
Book ChapterDOI
1.129 – Engineering the Biophysical Properties of Basement Membranes into Biomaterials: Fabrication and Effects on Cell Behavior
TL;DR: It is demonstrated that biophysical cues are essential components to be included in the design of cell studies for tissue engineering and can elicit different cell responses compared to micron-scale topography.
Journal ArticleDOI
Bidirectional Mechanical Response Between Cells and Their Microenvironment
TL;DR: In this paper, the authors showed that there exists a direct mechanical interplay between cells and their microenvironment in both directions, where both elements can be mechanically altered by one another.
Journal ArticleDOI
Microenvironment Stiffness Amplifies Post-ischemia Heart Regeneration in Response to Exogenous Extracellular Matrix Proteins in Neonatal Mice.
TL;DR: In this paper, the authors showed that cross-transplantation of decellularized extracellular matrix (dECM) can stimulate regeneration in myocardial infarct (MI) models.
Posted ContentDOI
Biphasic mechanosensitivity of TCR mediated adhesion of T lymphocytes
TL;DR: It is shown that T cells adhering via the TCR-complex respond to environmental stiffness in an unusual biphasic fashion, as the stiffness increases, adhesion and spreading first increase, then decrease, attaining their maximal values on an optimally stiff surface.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
Journal ArticleDOI
Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
Journal ArticleDOI
Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.