Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Cell Type-Dependent Integrin Distribution in Adhesion and Migration Responses on Protein-Coated Microgrooved Substrates
Adria Sales,Karen Ende,Jennifer Diemer,Adriana R. Kyvik,Jaume Veciana,Imma Ratera,Ralf Kemkemer,Ralf Kemkemer,Joachim P. Spatz,Joachim P. Spatz,Judith Guasch +10 more
TL;DR: The influence of surface topography and chemistry on αvβ3 and α5β1 integrin-mediated cell adhesion and migration of healthy and malignant human cell types was studied, using microgrooved and flat substrates covered by two different extracellular proteins, fibronectin and vitronect in this work.
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Nonlinear Cellular Mechanical Behavior Adaptation to Substrate Mechanics Identified by Atomic Force Microscope.
TL;DR: The effect of substrate mechanics on the nonlinear biomechanical behavior of living cells was investigated using indentation-based atomic force microscopy and it was found that living cells sense and adapt to substrate mechanics that were adapted to the substrates’ nonlinear mechanics.
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Oxygen Plasma Etching of Silk Fibroin Alters Surface Stiffness: A Cell-Substrate Interaction Study
TL;DR: In vitro results revealed that the increased stiffness of plasma-treated SF affected only L 929 adhesion, not hMSC, and L929 cell attachment and spreading were better on the stiffer surface than the untreated surface, while h MSC could spread well on all SF surfaces.
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Differential β3 Integrin Expression Regulates the Response of Human Lung and Cardiac Fibroblasts to Extracellular Matrix and Its Components.
Nick Joseph Merna,Kelsey Michiko Fung,Jean J. Wang,Cristi R. King,Kirk C. Hansen,Karen L. Christman,Steven C. George +6 more
TL;DR: In this article, the structure and composition of extracellular matrix (ECM) derived from whole organ decellularization has been successfully used in a variety of tissue engineering applications.
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Molecular Organization of Integrin-Based Adhesion Complexes in Mouse Embryonic Stem Cells
TL;DR: The results suggest that while core structural and mechanosensitive elements are operational in mESC FAs, their structural organization and regulatory aspects may diverge significantly from "classical" F as, which may account for the attenuated mechanobiological responses of these cell types.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.