Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Book ChapterDOI
Mechanotransduction, Metastasis and Genomic Instability
TL;DR: The ways in which physical attributes of the tumor microenvironment can promote metastasis and genomic instability, two hallmark features of cancer are discussed.
Journal ArticleDOI
Advancing bone tissue engineering one layer at a time: a layer-by-layer assembly approach to 3D bone scaffold materials.
Mohammad Sahebalzamani,Monika Ziminska,Helen O. McCarthy,Tanya J. Levingstone,Nicholas Dunne,Andrew Hamilton +5 more
TL;DR: In this paper , a review of the state of the art of the layer-by-layer assembly technique, and the properties and functions of LbL-assembled films for engineered bone scaffold application, combination of multilayers for multifunctional coatings and recent advancements in the application of lbL assembly in bone tissue engineering.
Journal ArticleDOI
Multi-scale mechanical characterization of prostate cancer cell lines: Relevant biological markers to evaluate the cell metastatic potential.
J. Zouaoui,Ana-Maria Trunfio-Sfarghiu,L. Brizuela,Agnès Piednoir,Ofelia Maniti,B. Munteanu,Saida Mebarek,Agnès Girard-Egrot,Ahmed Landoulsi,Thierry Granjon +9 more
TL;DR: Differences in viscoelasticity between PC-3 and WPMY-1 cells are attributed to modifications of both elements, which may be one of the diverse mechanisms that cancer cells adopt to cope with the various physiological conditions that they encounter.
Journal ArticleDOI
Precise positioning of cancerous cells on PDMS substrates with gradients of elasticity.
TL;DR: Results show that cancerous cells proliferate significantly more effective on soft PDMS, whereas the stiff one is almost cell-repellant, which strong impact of substrate elasticity on cellular behavior is driving force enabling precise positioning of cells.
Journal ArticleDOI
Two-input protein logic gate for computation in living cells.
Yashavantha L. Vishweshwaraiah,Jiaxing Chen,Venkat R. Chirasani,Erdem Tabdanov,Nikolay V. Dokholyan +4 more
TL;DR: In this paper, an engineered, single protein design that is allosterically regulated to function as a "two-input logic OR gate" was presented, based on chemo-and optogenetic regulation of focal adhesion kinase.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
Journal ArticleDOI
Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
Journal ArticleDOI
Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.