Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Universal kinetics for engagement of mechanosensing pathways in cell adhesion
TL;DR: This work studies the population dynamics of spreading cells, investigating the characteristic processes involved in cell response to the substrate, and shows that unlike the individual cell morphology, this population dynamics does not depend on the substrate stiffness.
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Biomechanical control of lysosomal secretion via the VAMP7 hub: a tug-of-1 war mechanism between VARP and LRRK1
Guan Wang,Sebastien Nola,Sebastien Nola,Simone Bovio,Maïté Coppey-Moisan,Frank Lafont,Thierry Galli,Thierry Galli +7 more
TL;DR: A mechanism whereby biomechanical constraints regulate VAMP7- dependent lysosomal secretion via LRRK1 and VARP tug-of-war control of the peripheral readily- releasable pool of secretory lysOSomes is proposed.
Substrate rigidity regulates fibronectin matrix assembly in fibroblasts
TL;DR: Analysis of matrix assembled by cells grown on FN-coated polyacrylamide gels of varying stiffnesses showed that rigid substrates stimulate FN matrix assembly, whereas assembly is inhibited on softer substrates.
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Cells immersed in collagen matrices show a decrease in plasma membrane fluidity as the matrix stiffness increases.
TL;DR: In this paper , the effect of matrix stiffness on the distribution of membrane lipid domains in NIH-3 T3 cells immersed in matrices of varying concentrations of collagen type I, for 24 or 72 h.
Impact of Extracellular Matrix Structure and Integrin Expression on Human Fibroblast Phenotype
TL;DR: It is indicated that ECM stiffness strongly correlates with collagen and elastin alignment in the ECM following decellularization, which can potentially impact fibroblast collagen and α-SMA expression during recellularization.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.