Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Cytoskeletal control of growth and cell fate switching.
TL;DR: Recent work has revealed that mechanical tension-dependent changes in cell shape and cytoskeletal structure are equally critical for control of growth, as well as cell fate switching.
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Cell adhesion strength is controlled by intermolecular spacing of adhesion receptors
Christine Selhuber-Unkel,Thorsten Erdmann,Monica Lopez-Garcia,Horst Kessler,Ulrich S. Schwarz,Joachim P. Spatz +5 more
TL;DR: The results show that cells can amplify small differences in adhesive cues to large differences in cell adhesion strength, and the weak link between the intra- and extracellular space is at the intracellular side of a focal contact.
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Emergent complexity of the cytoskeleton: from single filaments to tissue
Florian Huber,Jörg Schnauß,Susanne Rönicke,Philipp Rauch,Karla Müller,C. Fütterer,Josef A. Käs +6 more
TL;DR: This review aims at introducing the physics of the cytoskeleton ranging from single biopolymer filaments to multicellular organisms, with the focus on the intertwined nature of the different physical scales that give rise to numerous emergent properties by means of self-organization or self-assembly.
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Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity.
Abhishek Kumar,Mingxing Ouyang,Koen van den Dries,Ewan J. McGhee,Keiichiro Tanaka,Marie D. Anderson,Alex Groisman,Benjamin T. Goult,Kurt I. Anderson,Martin A. Schwartz +9 more
TL;DR: A novel talin sensor is used to measure talin tension and provide insights into focal adhesion force transmission and mechanosensitivity in integrins to the actin cytoskeleton.
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Rho GTPases Mediate the Mechanosensitive Lineage Commitment of Neural Stem Cells
Albert J. Keung,Elena M. de Juan-Pardo,Elena M. de Juan-Pardo,David V. Schaffer,David V. Schaffer,Sanjay Kumar +5 more
TL;DR: In this article, the authors demonstrate that extracellular matrix (ECM) derived mechanical signals act through Rho GTPases to activate the cellular contractility machinery in a key early window during differentiation to regulate NSC lineage commitment.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.