Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Tunable 3D Agarose-Well to enhance structural integrity of a reconstructed human skin equivalent
TL;DR: The agarose-fibrils in the well, assembled with penetrated collagen in dermis layer were proved to enhance the structural integrity and further regulate the mechanical strength from 6.8 kPa to 20.9 kPa.
Chapter 6 On the Application of Multiphasic Theories to the Problem of Cell- substrate Mechanical Interactions
TL;DR: After discussing the general formalism based on balance laws and constitutive relation, the chapter introduces a numerical strategy, based on the extended finite element, to capture the contraction of cells on compliant substrate.
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AFM and Nanoindentation Studies of Bone Nodules on Chitosan-Polygalacturonic Acid-Hydroxyapatite Nanocomposites
TL;DR: Overall, CPHfilms provide a favorable microenvironment for cell organization and bone noduleregeneration that regulates the mechanical behavior of cell-substrate interactions.
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Substrate elasticity modulates TGF beta stimulated re-differentiation of expanded human articular chondrocytes
Daniel Vonwil,Andreas Trüssel,Olivia Haupt,Samy Gobaa,Andrea Barbero,V. Prasad Shastri,Ivan Martin +6 more
TL;DR: Substrate elasticity modulated the TGF-β1 stimulated re-differentiation of EDD HAC and Mechanical compliance is thus an important parameter to be coupled with the delivery of appropriate morphogens in designing biomaterials for cartilage engineering and repair.
Posted ContentDOI
Mouse T cell priming is enhanced by maturation-dependent stiffening of the dendritic cell cortex
TL;DR: Stiffness responses are most robust when T cells are stimulated with pMHC rather than anti-CD3ε, consistent with a mechanosensing mechanism involving receptor deformation.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.