Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Book ChapterDOI
4.5 Mechanical Properties of Actin Networks
Karen E. Kasza,David A. Weitz +1 more
TL;DR: Measuring the rheology of and forces generated by actin networks provides insight into the physical mechanisms that underlie the elasticity of crosslinked networks and the behavior of contractile and protrusive networks, both in vitro and in cells.
DissertationDOI
Single Cell Pico Force Microscopy: A Novel Tool for High Resolution Measurement of Cell Forces
TL;DR: A systematic effort to build a library of molecular-mechanical force signatures of various common cytoskeleton reactions and an effort to stimulate and observe compliance sensing and response in adherent cells are proposed.
Biomechanics of Idiopathic Pulmonary Fibrosis and Inferior Vena Cava Filter Perforation
TL;DR: The author reveals that the author’s motivation to write the book was motivated by a love of science and a desire to contribute towards the humanizing of science.
Dissertation
Effets de la macro-architecture du substrat sur l'activité et la différenciation des ostéoblastes
TL;DR: In this paper, the role of the macroarchitecture du substrat on the comportement of the cellules is investigated and the effect of the geometries of the substrate on the cellulae is investigated.
Journal ArticleDOI
A strategy to quantify myofibroblast activation on a continuous spectrum
TL;DR: In this paper , the authors developed a strategy based on microscopy imaging and machine learning methods to quantify myofibroblasts activation in vitro on a continuous scale, and compared these results to a distribution of cell phenotypes generated from transcriptomic data using single-cell RNA sequencing.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
Journal ArticleDOI
Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
Journal ArticleDOI
Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.