Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Survivin is a mechanosensitive cell cycle regulator in vascular smooth muscle cells
John C. Biber,Andra Sullivan,Joseph Brazzo,Amanda Krajnik,Yun Heo,Kerry E. Poppenberg,Vincent M. Tutino,Su Jin Heo,John Kolega,Kwonmoo Lee,Yongho Bae +10 more
TL;DR: In this paper , the authors found that survivin, a member of the inhibitor of apoptosis protein family, mediates stiffness-sensitive cell cycling and proliferation in vivo and in vitro as determined by gene network and pathway analyses.
Journal ArticleDOI
How a Single Cell Sense its Mechanical Environment
Shengmao Lin,Linxia Gu +1 more
TL;DR: This work has shown that cell contractions, generated by the cross-bridging interaction of actin and myosin II motors, maintain a tensional homeostasis in response to mechanical disturbance, and investigates the role of local tension in this response.
Journal ArticleDOI
Cell-Specific Response of NSIP- and IPF-Derived Fibroblasts to the Modification of the Elasticity, Biological Properties, and 3D Architecture of the Substrate
Natalia Janiszewska,Barbara Orzechowska,Kamil Awsiuk,Jakub Rysz,Svitlana Tymetska,Joanna Raczkowska +5 more
TL;DR: In this paper , the authors investigated the effect of biological, physical, and topographical modification of the substrate on the properties of IPF-and nonspecific interstitial pneumonia (NSIP) derived fibroblasts and searched for the parameters enabling their identification.
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Soft drug-resistant ovarian cancer cells invade via two distinct mechanisms utilizing myosin IIB
Aastha Kapoor,Bhushan Thakur,Melissa Monteiro,Alakesh Das,Sejal Desai,Snehal M. Gaikwad,Amirali B. Bukhari,Pankaj Mogha,Abhijit Majumder,Abhijit De,Pritha Ray,Shamik Sen +11 more
TL;DR: It is shown that cisplatin-resistant, paclitaxel-resistant and dual drug-resistant OCCs are softer and more contractile than drug-sensitive cells, and the role of myosin IIB in regulating both modes of invasion.
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Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D Cultures.
TL;DR: This review provides the background on fluorescent molecular rotors, which are attractive tools due to the relationship of their emission quantum yield with environmental microviscosity and makes the case for their use in both 2D and 3D cell cultures and speculate on their fundamental and practical applications in cell biology.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
Journal ArticleDOI
Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.