Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Enzymatic cross-linking of collagens in organ fibrosis - resolution and assessment
Martin Pehrsson,Joachim Høg Mortensen,Tina Manon-Jensen,Anne-Christine Bay-Jensen,Morten A. Karsdal,Michael J. Davies +5 more
TL;DR: In this article, the authors discussed potential biomarkers specifically quantifying proteolytic fragments of collagen cross-linking, which may aid in the development and proper assessment of fibrosis resolution in coming years.
Journal ArticleDOI
Fabrication of elastomer pillar arrays with elasticity gradient for cell migration, elongation and patterning.
TL;DR: A novel yet straightforward method to fabricate elastomeric micropillar substrates with different height gradients to study the effect of substrate elasticity on stem cell behavior and differentiation and showed that when the cell density was sufficiently high, cells tend to be oriented in the same direction locally which was affected by both underneath pillars and cell-cell contact.
Journal ArticleDOI
SLIT3 deficiency attenuates pressure overload-induced cardiac fibrosis and remodeling.
Lianghui Gong,Lianghui Gong,Shuyun Wang,Li Shen,Catherine Z. Liu,Mena Shenouda,Baolei Li,Xiaoxiao Liu,John A. Shaw,Alan L Wineman,Yifeng Yang,Dingding Xiong,Anne Eichmann,Anne Eichmann,Sylvia M. Evans,Sylvia M. Evans,Stephen J. Weiss,Ming-Sing Si +17 more
TL;DR: Results indicate that SLIT3 is important for regulating fibroblast activity and fibrillar collagen synthesis in an autocrine manner, making it a potential therapeutic target for fibrotic diseases, especially myocardial fibrosis and adverse remodeling induced by persistent afterload elevation.
Journal ArticleDOI
Effect of Electrospun Fiber Mat Thickness and Support Method on Cell Morphology.
Mark A Calhoun,Sadiyah Sabah Chowdhury,Mark Tyler Nelson,John J. Lannutti,Rebecca B. Dupaix,Jessica O. Winter +5 more
TL;DR: The results suggest that EFM curvature may play a role in cell response, separate from Young’s modulus, possibly because of internal tension generated and could improve scaffold performance and ultimately patient outcomes.
BookDOI
Analysis and Design of Biological Materials and Structures
TL;DR: In this article, the biomechanical behavior of C3-C5 porcine cervical vertebrae is analyzed using three numerical procedures when a compressive load is applied.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
Journal ArticleDOI
Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.