Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Mechanical and Morphological Analysis of Cancer Cells on Nanostructured Substrates
TL;DR: Comparing the morphology, proliferation, spreading, and stiffness of highly aggressive glioblastoma multiforme cancer cells and normal fibroblast cells seeded on a variety of ordered polymeric nanostructures (nanopillars and nanochannels) shows that gliOBlastomas are softer, spread to a larger area, and elongate less than fibroblasts.
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Development of a novel liquid crystal based cell traction force transducer system
TL;DR: A new technique involving cytochalasin-B treatment was used to disrupt the intracellular force generating actin fibers, and consequently the biaxial strain in the LC induced by the cells was determined and a wide range of CTFs was determined, found to be linearly proportional to the length of the deformations.
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Substrate topography interacts with substrate stiffness and culture time to regulate mechanical properties and smooth muscle differentiation of mesenchymal stem cells.
Azim Parandakh,Azadeh Anbarlou,Mohammad Tafazzoli-Shadpour,Abdolreza Ardeshirylajimi,Mohammad-Mehdi Khani +4 more
TL;DR: Substrate stiffness and topography are two powerful means by which mesenchymal stem cells (MSCs) activities can be modulated and can be helpful in the mechano-regulation of MSCs for vascular tissue engineering applications.
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The effect of set point ratio and surface Young’s modulus on maximum tapping forces in fluid tapping mode atomic force microscopy
TL;DR: In this article, the impact of the Young's modulus of the surface on the maximum tapping force was explored in fluid TMAFM via simulation and experiment, where the numerical AFM model contains a feedback loop, allowing for the simulation of the entire scanning process.
Patent
Enhancement of skeletal muscle stem cell engraftment by dual delivery of VEGF and IGF-1
David J. Mooney,Cristina Borselli,Herman H. Vandenburgh,Eduardo A. Silva,I Lin Wang,Dmitry Shvartsman,Hannah Storrie,Jeff Lichtman +7 more
TL;DR: An improved device and method for extended repair and regeneration of muscle tissue is described in this paper, which consists of a scaffold comprising an ECM component, a combination of growth factors such as VEGF and IGF, and a population of myogenic cells.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.