Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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DissertationDOI
Evaluating the use of laser processing and polishing techniques to generate micro-patterned surfaces for controlling fibroblast cell behaviour
TL;DR: In this article, the authors used micro and nano polymer substrates through different surface patterning techniques to compare their effect on human lung fibroblast (LL24) and bovine aorta endothelium (BAE-1) cell behaviors.
Journal ArticleDOI
Morphology and cellular-traction of fibroblasts on 2D silk-fibroin hydrogel substrates
TL;DR: Results suggest that surface-stiffness of SF-hydrogel, rather than nature of surface-ligand, regulates both cellular morphology and cellular traction stresses.
Posted ContentDOI
Vimentin Intermediate Filaments Can Enhance or Abate Active Cellular Forces in a Microenvironmental Stiffness-Dependent Manner
Farid Alisafaei,K K Mandal,Maxx Swoger,Haiqian Yang,Ming-feng Guo,Paul A. Janmey,Alison E. Patteson,Vivek B. Shenoy +7 more
TL;DR: Vimentin is a marker of the epithelial to mesenchymal transition which takes place during important biological processes including embryogenesis, metastasis, tumorigenesis, fibrosis, and wound healing and it is shown that vimentin impacts cellular forces in a matrix stiffness-dependent manner.
Journal ArticleDOI
Ventral stress fibers induce plasma membrane deformation in human fibroblasts
TL;DR: In this article, the authors observed fluorescent structures in myofibroblasts labeled with cytosolic fluorophores and revealed these structures to be ridges in the plasma membrane caused by ve...
Posted ContentDOI
Human Mammary Cells in a Mature, Stratified Epithelial Layer Flatten and Stiffen Compared to Confluent and Single Cells
TL;DR: The changes in stiffness, morphology and actin distribution of human mammary epithelial cells (HMECs) as they transition from single cells to confluency to a mature epithelial layer are determined using a combination of atomic force, fluorescence and confocal microscopy.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
Journal ArticleDOI
Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
Journal ArticleDOI
Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.