Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Cytoskeletal remodeling induced by substrate rigidity regulates rheological behaviors in endothelial cells.
TL;DR: To characterize the viscoelastic behavior of endothelial cells, cultivated on variably compliant substrates, fuzzy c-means clustering algorithm was applied to partition elastic data into biologically meaningful groups, representative of different regions in cells.
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The Mechanical Microenvironment in Breast Cancer
TL;DR: Mechanotransduction is the interpretation of physical cues by cells through mechanosensation mechanisms that elegantly translate mechanical stimuli into biochemical signaling pathways, which support three mechanical stressors as mechanical modifiers in breast cancer.
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Quantitative mechanical analysis of indentations on layered, soft elastic materials.
TL;DR: It is found that the effect of the underlying substrate can be quantitatively predicted by the mismatch in elastic moduli and the homogeneous-case contact radius relative to the layer height for all tested probe geometries.
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Elastic-Fluid Model for DNA Damage and Mutation from Nuclear Fluid Segregation Due to Cell Migration.
Rachel R. Bennett,Charlotte R. Pfeifer,Jerome Irianto,Yuntao Xia,Dennis E. Discher,Andrea J. Liu +5 more
TL;DR: A model for a mechanism by which nuclear deformation can lead to DNA damage is proposed and it is shown that squeeze-out of the fluid, and hence of the mobile repair factors, is sufficient to account for the extent of DNA damage and genomic variation observed experimentally.
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Cryo DualBeam Focused Ion Beam–Scanning Electron Microscopy to Evaluate the Interface Between Cells and Nanopatterned Scaffolds
Edwin Lamers,X. Frank Walboomers,Maciej Domanski,George McKerr,Barry O'Hagan,Clifford Barnes,Lloyd Peto,Regina Luttge,Louis Winnubst,Han Gardeniers,John A. Jansen +10 more
TL;DR: Cryo DualBeam focused ion beam-scanning electron microscopy (FIB-SEM) was used as a novel approach to observe the interactions between frozen hydrated cells and nanometric structures in high detail to evaluate processes that take place at the interface between tissue and substrate.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.