Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Enhanced Biomechanically Mediated “Phagocytosis” in Detached Tumor Cells
TL;DR: In this paper, a self-designed 3D-printing apparatus is introduced to simulate a floating environment for cell incubation, which enables constant stirring and facilitates cell uptake of inert particles.
Journal ArticleDOI
Cell mediated remodeling of stiffness matched collagen and fibrin scaffolds
TL;DR: In this paper , the authors measured how stiffness around dermal fibroblasts and human fibrosarcoma HT1080 cells differs with concentration of rat tail type 1 collagen (T1C) and type of ECM.
Journal ArticleDOI
Small-scale displacement fluctuations of vesicles in fibroblasts
TL;DR: In this article, the authors show that myosin-II activity increases the effective diffusivity of vesicles and its inhibition facilitates longer states of non-directed motion.
Proceedings ArticleDOI
How deeply cells feel
TL;DR: It is shown that cells increasingly respond to the rigidity of an underlying `hidden' surface starting at about 10-20 μm gel thickness with a characteristic tactile length of less than about 5 μm, by utilizing polyacrylamide hydrogels with various thickness.
Journal ArticleDOI
Aquatic flower-inspired cell culture platform with simplified medium exchange process for facilitating cell-surface interaction studies
TL;DR: A novel aquatic flower-inspired cell culture platform (AFIP) is presented, devised to capture and dispense cell culture medium based on interactions between an elastic polymer substrate and a liquid medium, and demonstrated that AFIP can be applied to the cell-surface interaction studies as an alternative to the conventional method.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
Journal ArticleDOI
Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
Journal ArticleDOI
Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.