Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Book ChapterDOI
Structural, Mechanical and Functional Properties of Intermediate Filaments from the Atomistic to the Cellular Scales
TL;DR: Joint experimental, computational and theoretical multiscale studies of the mechanical properties of intermediate filament proteins, an important component in the cell’s cytoskeleton and a key player in cell mechanics, migration and disease processes including cancer are reviewed.
Journal ArticleDOI
Substrate Stiffness Mediated Metastasis Like Phenotype of Colon Cancer Cells is Independent of Cell to Gel Adhesion
M. Yakut Ali,M. Taher A. Saif +1 more
TL;DR: Results suggest that MLP of HCT-8 cells on PA gels is independent of cell to gel adhesion in 2D in vitro culture.
Journal ArticleDOI
Spatial correlation of cell stiffness and traction forces in cancer cells measured with combined SICM and TFM
TL;DR: The first direct combination of scanning ion conductance microscopy and traction force microscopy is presented, which is used to identify a correlation between the local stiffness and the local traction force density in living cells, which indicates that the interplay between cell stiffness and traction forces is altered in cancer cells as compared to healthy cells.
Journal ArticleDOI
Active stiffening of F-actin network dominated by structural transition of actin filaments into bundles
TL;DR: It is demonstrated through computational models that the stretching of cross-linkers and molecular motors has less impact on the active stiffening, while it is more sensitive to cytoskeleton reorganization during the elasticity sensing.
Journal ArticleDOI
Evaluation of cellular adhesion and organization in different microporous polymeric scaffolds
TL;DR: This study optimized the surface treatment, pore size, and choice of scaffold material with respect to cellular adhesion, tissue organization, and expression of complex physiologically relevant (CPR) outcomes such as the presence of bile canaliculi‐like structures.
References
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Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.