Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
More filters
Journal ArticleDOI
A guide to mechanobiology: Where biology and physics meet.
Karin A. Jansen,Dominique Donato,Hayri E. Balcioglu,Thomas Schmidt,Erik H.J. Danen,Gijsje H. Koenderink +5 more
TL;DR: An introductory overview of the key players important to cellular mechanobiology is provided, taking a biophysical perspective and focusing on a comparison between flat versus three dimensional substrates.
Journal ArticleDOI
Fibronectin fibrillogenesis regulates three-dimensional neovessel formation
Xiaoming Zhou,R. Grant Rowe,Nobuaki Hiraoka,Jerry P. George,Denis Wirtz,Deane F. Mosher,Ismo Virtanen,Michael A. Chernousov,Stephen J. Weiss +8 more
TL;DR: It is demonstrated that endothelial cells initiate neovascularization by unfolding soluble fibronectin (Fn) and depositing a pericellular network of fibrils that serve to support cytoskeletal organization, actomyosin-dependent tension, and the viscoelastic properties of the embedded cells in a 3D-specific fashion.
Journal ArticleDOI
Viscoelastic hydrogels for 3D cell culture.
TL;DR: The characterization and molecular basis of hydrogel viscoelasticity and plasticity, and newly developed approaches to tuning viscoELasticity in hydrogels for 2D and 3D culture are described.
Journal ArticleDOI
The nano-scale mechanical properties of the extracellular matrix regulate dermal fibroblast function.
Volker Achterberg,Lara Buscemi,Heike Diekmann,Josiane Smith-Clerc,Helge Schwengler,Jean-Jacques Meister,Horst Wenck,Stefan Gallinat,Boris Hinz +8 more
TL;DR: To establish elastic culture substrates that reproduce the variable softness of dermis, Young's elastic modulus E of human dermis is determined at the cell perception level using atomic force microscopy to generate fibroblasts that are more suitable to investigate physiologically relevant cell processes.
Journal ArticleDOI
Pushing Back: Wound Mechanotransduction in Repair and Regeneration
TL;DR: An improved understanding of these interactions will facilitate the development of novel biophysical materials and mechanomodulatory approaches to augment wound repair and regeneration.
References
More filters
Journal ArticleDOI
Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
Journal ArticleDOI
Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
Journal ArticleDOI
Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.