Fibroblast Adaptation and Stiffness Matching to Soft Elastic Substrates
TLDR
Within a range of stiffness spanning that of soft tissues, fibroblasts tune their internal stiffness to match that of their substrate, and modulation of cellular stiffness by the rigidity of the environment may be a mechanism used to direct cell migration and wound repair.About:
This article is published in Biophysical Journal.The article was published on 2007-12-15 and is currently open access. It has received 999 citations till now. The article focuses on the topics: Stiffness.read more
Citations
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Engineering strategies to mimic the glioblastoma microenvironment
TL;DR: The regulation of GBM tumors by cell-extrinsic factors, collectively termed the microenvironment, include the extracellular matrix, blood vessels, stromal cells that surround tumor cells, and all associated soluble and scaffold-bound signals are described.
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Stem cell therapy without the cells.
TL;DR: Great strides have been made in the development of stem cell therapies outside of academic circles, such as the use of purified stem cells instead of whole bone marrow transplants in cancer patients, where physicians avoid re-injecting the patients with their own cancer cells.
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Innovative Tools for Mechanobiology: Unraveling Outside-In and Inside-Out Mechanotransduction.
Danahe Mohammed,Marie Versaevel,Céline Bruyère,Laura Alaimo,Marine Luciano,Eléonore Vercruysse,Anthony Procès,Sylvain Gabriele +7 more
TL;DR: The basic conceptual fundamentals related to cell mechanobiology are presented and the current state-of-the-art technologies that facilitate the understanding of mechanotransduction signaling pathways are reviewed.
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A photo-modulatable material for probing cellular responses to substrate rigidity
TL;DR: A modulatable hydrogel by reacting linear polyacrylamide with a photosensitive crosslinker allows UV-mediated control of rigidity, softening by 20-30% upon irradiation at a dose tolerated by live cells, indicating that rigidity sensing is localized to the frontal region.
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Microglia mechanics: immune activation alters traction forces and durotaxis.
Lars Bollmann,Lars Bollmann,David E. Koser,David E. Koser,Rajesh Shahapure,Hélène O. B. Gautier,Gerhard Holzapfel,Giuliano Scarcelli,Malte C. Gather,Elke Ulbricht,Kristian Franze +10 more
TL;DR: A mathematical model connecting traction forces with the durotactic behavior of migrating microglial cells is developed, demonstrating that microglia are susceptible to mechanical signals, which could be important during central nervous system development and pathologies.
References
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Matrix elasticity directs stem cell lineage specification.
TL;DR: Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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Tensional homeostasis and the malignant phenotype.
Matthew J. Paszek,Nastaran Zahir,Kandice R. Johnson,Johnathon N. Lakins,Gabriela I. Rozenberg,Amit Gefen,Cynthia A. Reinhart-King,Susan S. Margulies,Micah Dembo,David Boettiger,Daniel A. Hammer,Valerie M. Weaver +11 more
TL;DR: It is found that tumors are rigid because they have a stiff stroma and elevated Rho-dependent cytoskeletal tension that drives focal adhesions, disrupts adherens junctions, perturbs tissue polarity, enhances growth, and hinders lumen formation.
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Cell Movement Is Guided by the Rigidity of the Substrate
TL;DR: It is discovered that changes in tissue rigidity and strain could play an important controlling role in a number of normal and pathological processes involving cell locomotion, including morphogenesis, the immune response, and wound healing.
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Cell locomotion and focal adhesions are regulated by substrate flexibility
Robert J. Pelham,Yu-li Wang +1 more
TL;DR: The ability of cells to survey the mechanical properties of their surrounding environment is demonstrated and the possible involvement of both protein tyrosine phosphorylation and myosin-generated cortical forces in this process is suggested.